Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection
文献类型： 外文期刊
作者： Guo, Yu 1 ; Cheng, Jing 2 ; Zhang, Shuai 1 ; Zhang, Yuanyuan 2 ; Zuo, Yuzhu 1 ; Liu, Tao 1 ; Wang, Yun 1 ; Yang, Chun 2 ; Cheng, Chunjie 2 ; Fan, Jinghui 1 ; Jiang, Haijun 2 ;
作者机构： 1.Agr Univ Hebei, Coll Vet Med, Baoding, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing, Peoples R China
3.Baoding Vocat & Tech Coll, Baoding, Peoples R China
关键词： avian metapneumonovirus/C; enhance green fluorescent protein; HA protein; immune protection
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）
ISSN：
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： To prevent H9N2 avian influenza virus (AIV) and Avian metapneumonovirus/C (aMPV/C) infections, we constructed recombinant aMPV/C viruses expressing the HA protein of H9N2 AIV. In addition, EGFP was inserted into the intermediate non-coding region of P-M protein in the aMPV/C genome using a reverse genetic system. The conditions for rescuing the recombinant virus were enhanced followed by insertion of the H9N2 AIV HA gene into the same location in the aMPV/C. The constructed recombinant virus raMPV/C-HA expressed the H9N2 AIV HA protein and showed good stability. Immunization of chicks with raMPV/C-HA increased the generation of neutralizing antibodies against aMPV and H9N2 AIV for 21 days. In the late challenge experiment, raMPV/C-HA effectively inhibited the replication of the virus in vivo, decreased the incidence of infection and conferred protection effects.
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