您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Multiple crRNAs-assisted CRISPR/Cas12a assay targeting cytochrome b gene for amplification-free detection of meat adulteration

文献类型： 外文期刊

作者： Chen, Yanju 1 ; Yang, Tianyi 1 ; Qian, Siwenjie 1 ; Peng, Cheng 2 ; Wang, Xiaofu 2 ; Wang, Tingzhang 4 ; Che, Yang 4 ; Ji, Feng 3 ; Wu, Jian 1 ; Xu, Junfeng 2 ;

作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Zhejiang Univ, Affiliated Hosp 1, Sch Med, Hangzhou 310003, Peoples R China

4.Zhejiang Inst Microbiol, Key Lab Microbiol Technol & Bioinformat Zhejiang P, Hangzhou 310012, Peoples R China

5.ZJU Hangzhou Global Sci & Technol Innovat Ctr, 733,Jianshe 3rd Rd, Hangzhou 311200, Peoples R China

关键词： Clustered regularly interspaced short; palindromic repeats; Multiple crRNAs; Amplification -free; Cytochrome b; Food adulteration

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.911； 五年影响因子：6.467 ）

ISSN： 0003-2670

年卷期： 2022 年 1231 卷

页码：

收录情况： SCI

摘要： Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems have been widely applied in nucleic acid analysis for the high specificity. Coupled with pre-amplification steps, the sensitivity of CRISPR-based detection is greatly improved. However, an extra pre-amplification step not only complicates the detec-tion procedures but may also cause aerosol contaminations in the process of transferring amplified solution into CRISPR system. In this study, we demonstrate that combination of multiple crRNAs in CRISPR/Cas12a system can enhance the detection sensitivity. Based on it, we establish a multiple crRNAs enhanced CRISPR (meCRISPR) method and apply it to meat adulteration identification. Take cytochrome b (Cyt b) gene as a target, meCRISPR method can directly detect as low as 1.13 ng/mu L extracted pork DNA and 5% (w/w) pork contamination in pork and beef meat mixtures. There is no cross-reaction with extracted chicken, beef, duck and fish DNA. meCRISPR reaction is incubated at an isothermal temperature, and the detection process can be completed in a designed portable apparatus with a heat block, a light emitting diode and filters. For the simplicity, specificity and sufficient sensitivity of meCRISPR method, it will have great prospects in species identification, food adultera-tion, and genetically modified food detection.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台