文献类型： 外文期刊

作者： Wang, Fei ¹ ; Li, Xiang ¹ ; Wang, Qi ¹ ; Jin, Qian ¹ ; Xu, Shujie ¹ ; Tang, Li ¹ ; Zeng, Zihan ¹ ; Fu, Aikun ¹ ; Zhu, Jinhui ³ ; Zhang, Qiao ⁴ ; Yang, Rongchang ⁵ ; Xiao, Yingping ² ; Li, Weifen ¹ ;

作者机构： 1.Zhejiang Univ, Inst Anim Nutr & Feed Sci, Coll Anim Sci, Key Lab Anim Mol Nutr,Educ Minist,Key Lab Anim Fee, Hangzhou 310058, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Zhejiang Univ, Affiliated Hosp 2, Dept Gen Surg, Sch Med, Hangzhou 310009, Peoples R China

4.Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Clin Pharm, Hangzhou 310003, Peoples R China

5.Murdoch Univ, Sch Agr Sci, Murdoch, WA 6150, Australia

6.Zhejiang Youheyhey Biotechnol Co LTD, Huzhou 313000, Zhejiang, Peoples R China

关键词： Lactic acid bacteria; Suanshui; Probiotic properties; TOPSIS analysis; Acute liver injury

期刊名称：PROBIOTICS AND ANTIMICROBIAL PROTEINS （ 影响因子：4.4； 五年影响因子：5.0 ）

ISSN： 1867-1306

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Suanshui is a traditional fermented vegetable food in the southwest of China that is a rich source of probiotics. This study aimed to screen probiotics from Suanshui that have a protective effect against liver injury using in vivo and in vitro experiments. Eleven strains were isolated according to acid-producing capacity and probiotic properties, including antibacterial activity against pathogenic bacteria, tolerance to artificial gastrointestinal fluid and bile salts, heat resistance, antioxidant capacity, hydrophobicity, autoaggregation capacity, and adhesion capacity. Three probiotic strains, LAB36 (Lacticaseibacillus paracasei, (L. paracasei)), LAB19 (Lactiplantibacillus plantarum (L. plantarum)), and LAB51 (Limosilactobacillus fermentum (L. fermentum)), were obtained by the TOPSIS comprehensive evaluation. Furthermore, in an in vitro model of inflammation induced by LPS treatment of AML12, metabolites derived from the intestinal contents of mice treated with the three strains significantly decreased the gene expression of IL-1 beta, TNF-alpha, and Caspase 3 in the LAB (19, 36, 51) + LPS group (P < 0.05) and significantly increased the gene expression of Bcl-2 compared with those in the untreated LPS group (P < 0.05). In addition, the LAB36 + LPS group also exhibited significantly decreased gene expression of TLR4 and Bax (P < 0.05). In vivo experiments, LAB36 pretreatment significantly decreased the levels of ALT, AST, and inflammatory factors (IL-1 beta and IL-6) in the serum, improved the antioxidant capacity, and reduced apoptosis in the livers of the model mice (liver injury induced by D-GalN/LPS). In summary, LAB36 isolated from Suanshui has a protective effect on liver injury.