A conserved viral amphipathic helix governs the replication site-specific membrane association
文献类型： 外文期刊
作者： Sathanantham, Preethi 1 ; Zhao, Wenhao 1 ; He, Guijuan 1 ; Murray, Austin 1 ; Fenech, Emma 3 ; Diaz, Arturo 4 ; Schuldiner, Maya 3 ; Wang, Xiaofeng 1 ;
作者机构： 1.Virginia Tech, Sch Plant & Environm Sci, Blacksburg, VA 24061 USA
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing, Peoples R China
3.Weizmann Inst Sci, Dept Mol Genet, Rehovot, Israel
4.La Sierra Univ, Dept Biol, Riverside, CA USA
期刊名称：PLOS PATHOGENS （ 影响因子：7.464； 五年影响因子：7.913 ）
ISSN： 1553-7366
年卷期： 2022 年 18 卷 9 期
页码：
收录情况： SCI
摘要： Positive-strand RNA viruses assemble their viral replication complexes (VRCs) on specific host organelle membranes, yet it is unclear how viral replication proteins recognize and what motifs or domains in viral replication proteins determine their destinations. We show here that an amphipathic helix, helix B in replication protein 1 a of brome mosaic virus (BMV), is necessary for la's localization to the nuclear endoplasmic reticulum (ER) membrane where BMV assembles its VRCs. Helix B is also sufficient to target soluble proteins to the nuclear ER membrane in yeast and plant cells. We further show that an equivalent helix in several plant- and human-infecting viruses of the Alsuviricetes class targets fluorescent proteins to the organelle membranes where they form their VRCs, including ER, vacuole, and Golgi membranes. Our work reveals a conserved helix that governs the localization of VRCs among a group of viruses and points to a possible target for developing broad-spectrum antiviral strategies.
- 相关文献
作者其他论文 更多>>
-
pH-responsive nanoparticles for oral delivery of RNAi for sustained protection against Spodoptera exigua
作者：Jiang, Yiping;Zong, Suman;Zhao, Jing;Xiao, Liubin;Xu, Dejin;Xu, Guangchun;Tan, Yongan;Wang, Xiaofeng;Zhu-Salzman, Keyan
关键词：Spodoptera exigua; RNAi; Pest management; Nanocarrier
-
Mesoporous silica nanoparticles enhance the toxicity of chlorantraniliprole to Spodoptera frugiperda by possibly inhibiting energy metabolism and chitin protein synthesis
作者：Zong, Suman;Xu, Dejin;Jiang, Yiping;Zhang, Xin;Zhao, Jing;Xiao, Liubin;Xu, Guangchun;Tan, Yongan;Zong, Suman;Ge, Linquan;Wang, Xiaofeng;Zhu-Salzman, Keyan;Zhang, Leigang;Gu, Aiguo;Hong, Hao
关键词：chlorantraniliprole; foliage adhesion; mesoporous silica nanoparticles; RNA-Seq;
Spodoptera frugiperda -
How to promote the application of green pesticides by farmers? Evolutionary game analysis based on "government-farmer-consumer"
作者：Wang, Xiaofeng;Cui, Xiaojun;Sun, Xiaolong
关键词：green pesticides; evolutionary game theory; user preference; regulatory intensity; ecological subsidies
-
Geminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infections
作者：Zhao, Wenhao;Ji, Yinghua;Zhou, Yijun;Zhao, Wenhao;Wang, Xiaofeng
关键词：
-
An oral dsRNA delivery system based on chitosan induces G protein-coupled receptor kinase 2 gene silencing for Apolygus lucorum control
作者：Qiao, Heng;Zhao, Jing;Xiao, Liubin;Xu, Dejin;Xu, Guangchun;Tan, Yongan;Qiao, Heng;Hao, Dejun;Wang, Xiaofeng;Zhu-Salzman, Keyan;Lei, Jiaxin
关键词：Apolygus lucorum; Chitosan nanoparticles; G protein-coupled receptor kinase 2; dsRNA delivery; RNA interference
-
A simplified RT-PCR assay for the simultaneous detection of tomato chlorosis virus and tomato yellow leaf curl virus in tomato
作者：Tu, Liqin;Wu, Shuhua;Gan, Shexiang;Zhao, Wenhao;Li, Shuo;Cheng, Zhaobang;Zhou, Yijun;Ji, Yinghua;Tu, Liqin;Zhu, Yuelin
关键词：Tomato chlorosis virus; Tomato yellow leaf curl virus; Simultaneous detection via RT-PCR
-
Development of a reverse-transcription loop-mediated isothermal amplification assay for the detection of Tobacco mild green mosaic virus (TMGMV)
作者：Zhao, Wenhao;Wu, Shuhua;Du, Linlin;Li, Tingfang;Cheng, Zhaobang;Zhou, Yijun;Ji, Yinghua
关键词：Tobacco mild green mosaic virus (TMGMV); Reverse-transcription loop-mediated; isothermal amplification (RT-LAMP); Virus detection; Pepper; Capsicum annuum
