Phylogenetic analysis of four Vibrio strains of pathogenic bacteria based on hemolysin genes and 16S rRNA genes
文献类型: 外文期刊
作者: Wang, Xiuli 1 ; Yuan, Ye 1 ; Wang, Hongyue 1 ; Zhou, Qunlan 2 ; Zhu, Jian 2 ; Chang, Yaqing 1 ; Cui, Yudong 3 ; Qiu, Xuem 1 ;
作者机构: 1.Dalian Fisheries Univ, Minist Agr, Key Lab Mariculture & Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisher Res Ctr, Wuxi 214081, Peoples R China
3.Heilongjiang August First Land Reclamat Univ, Coll Life Sci & Biotechnol, Daqing 163319, Peoples R China
关键词: Vibrio;hemolysin gene;16S rRNA gene;Phylogenetic analysis
期刊名称:2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11
ISSN:
年卷期: 2009 年
页码:
收录情况: SCI
摘要: In order to understand the phylogenetic relationships among Vibrio species more rationally, the hemolysin gene partial sequences of 4 Vibrio species (Vibrio alginolyticus, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio minius) were amplifyed and sequenced. We constructed two separate phylogenetic trees by using 16S rRNA and partial hemolysin gene sequences, respectively. The average similarities of hemolysin gene of Vibrio species was 37.2 %. Meanwhile, the average similarity of 16S rRNA gene of Vibrio species was 91.4 %. The variabilities of hemolysin gene in Vibrio is greater than that of 16S rRNA gene. By comparing the phylogenetic trees, the topology of hemolysin gene tree is almost consistent with that of 16S rRNA gene tree. Consequently, the hemolysin gene possesses the superiority in phylogenetic taxonomy of the genus Vibrio. Taxonomic classification of Vibrios with hemolysin gene instead of 16S rRNA gene was proved a better approach.
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