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Isolation and Characterization of Bovine RVA from Northeast China, 2017-2020

文献类型： 外文期刊

作者： Cheng, Xi 1 ; Wu, Wei 1 ; Teng, Fei 1 ; Yan, Yue 1 ; Li, Guiwei 2 ; Wang, Li 1 ; Wang, Xiaona 1 ; Wang, Ruichong 3 ; Zhou, Han 1 ; Jiang, Yanping 1 ; Cui, Wen 1 ; Tang, Lijie 1 ; Li, Yijing 1 ; Qiao, Xinyuan 1 ;

作者机构： 1.Northeast Agr Univ, Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Coll Vet Med, Dept Vet,Prevent Med, Harbin 150038, Peoples R China

2.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 161000, Peoples R China

3.Heilongjiang Prov Ctr Dis Control & Prevent, Dept Radiol Protect, Harbin 150030, Peoples R China

关键词： bovine rotavirus; epidemiological characteristics; virus isolation; sequence analysis

期刊名称：LIFE-BASEL （ 影响因子：3.251； ）

ISSN：

年卷期： 2021 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Group A rotaviruses (RVAs) are major enteric pathogens causing infections in calves. To investigate the epidemiological characteristics and genetic diversity of bovine rotavirus (BRV), 233 fecal samples were collected from calves with diarrhea in northeast China. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) and the outer capsid proteins VP7 and VP4 (G and P type, respectively) using RT-PCR. Ten of the 233 samples (4.3%) were identified as BRV positive and were used for virus isolation and sequence analysis, revealing that all strains analyzed were of the G6P[1] genotype. The isolates exhibited high VP6 sequence identity to the USA cow RVA NCDV strain (>99% amino acid identity) and were further shown to be closely related to Japanese cow RVA BRV101 and Israelian human RVA G6P[1] strains, with >99% amino acid identity to VP7 and VP4 proteins, respectively. Comparative analyses of genome-predicted amino acid sequences between the isolates and the NCDV strains indicated that the antigenicity and infectivity of the strains isolated had changed. In this study, BRV genotypes and the genetic diversity among vaccinated cattle herds were monitored to provide epidemiological data and references for early diagnosis, allowing for early detection of new, potentially pathogenic RVA strains.

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