您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of water-borne copper on the gills and hepatopancreas of Macrobrachium rosenbergii

文献类型： 外文期刊

作者： Li, Na 1 ; Ma, Qiang 1 ; Yang, Jian 2 ; Zhao, Yunlong 1 ; Brochon, Jean-Claude 3 ;

作者机构： 1.E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Ecol Environm & Resources Inland Fisherie, Wuxi, Peoples R China

3.Ecole Normale Super, Lab Biotechnol & Applicat Genet Pharmacol, Natl Res Ctr, Cachan, France

关键词： copper;gills;hepatopancreas;isozymes;Macrobrachium rosenbergii;polyacrylamide electrophoresis

期刊名称：INTEGRATIVE ZOOLOGY （ 影响因子：2.654； 五年影响因子：2.525 ）

ISSN： 1749-4877

年卷期： 2008 年 3 卷 4 期

页码：

收录情况： SCI

摘要： We focused on elucidating the toxic effects of water-borne copper on the giant freshwater prawn Macrobrachium rosenbergii. After seven days of exposure to copper (Cu2+) at concentrations ranging from 0.01 mg L-1 to 0.5 mg L-1, three isozymes, malate dehydrogenase, alkaline phosphatase and esterase, were analyzed and compared using polyacrylamide electrophoresis (PAGE) and biochemical staining. The results indicated that the electrophoretic patterns of the isozymes showed a copper-concentration-related difference. Low doses of copper stimulated strong expression of the three isozymes. Electrophoretic patterns of malate dehydrogenase and alkaline phosphatase gradually became weaker or even lost as the level of copper increased. In contrast, esterase patterns exhibited an increased molecular heterogeneity at higher copper concentrations. A transmission electron microscope was used to study ultrastructure differences in the gills and hepatopancreas of M. rosenbergii, and the results showed significant structural damage at increased levels of copper compared with the control group. The basement membranes and mitochondira in the gills were seriously damaged, the cuticle electron density distribution was not homogeneous, and an infolded basement membrane, circularized nucleus, disintegrated nuclear membrane, and decreased mitochondria number and size were observed in the gills. Similarly, flowing out of karyoplasms, partly falling microvilli, decreased mitochondrion, partly disappeared mitochondrial cristae, and a thinned matrix were observed in the hepatopancreas. These findings indicate that exposure to elevated copper levels might damage the ultrastructure of the gills and hepatopancreas of M. rosenbergii and might further weaken their normal physical activities. Isozymes were quite sensitive to environmental stress and changes in isozyme elctrophoretic patterns might be effective biomarkers of environmental contamination.

  • 相关文献

[1]Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Jia, Rui,Huo, Huan-Huan,Jia, Rui,Lei, Ji-Lin,Liu, Bao-Liang,Huang, Bin,Yin, Shu-Ting,Han, Cen.

[2]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[3]Molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster Pinctada fucata. Xu, Xinping,Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Ma, Jianjun,Jiang, Jingjing,Pan, Dequan,Zhang, Dianchang,Jiang, Jingjing.

[4]Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia. Sun, Shengming,Ge, Xianping,Fu, Hongtuo,Zhu, Jian,Xuan, Fujun,Zhang, Shiyong.

[5]Isolation and Characterization of a Ferritin cDNA from the Mud Crab Scylla paramamosain. Zhang, Dan,Jiang, Keji,Zhang, Fengying,Ma, Chunyan,Shi, Yanhong,Qiao, Zhenguo,Ma, Lingbo,Zhang, Dan.

[6]Dietary cholesterol-induced transcriptome differences in the intestine, hepatopancreas, and muscle of Oriental River prawn Macrobrachium nipponense. Gu, Xizhang,Fu, Hongtuo,Sun, Shengming,Gu, Xizhang,Fu, Hongtuo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi,Jiang, Sufei,Xiong, Yiwei,Jin, Shubo,Gong, Yongsheng,Wu, Yan.

[7]Genetic parameters and response to selection for harvest body weight of the giant freshwater prawn Macrobrachium rosenbergii. Luan, Sheng,Luo, Kun,Kong, Jie,Yang, Guoliang,Wang, Junyi,Zhang, Yufei,Gao, Qiang,Yang, Guoliang,Wang, Junyi,Zhang, Yufei,Gao, Qiang,Hu, Honglang. 2012

[8]Current status of the giant freshwater prawn ( Macrobrachium rosenbergii) industry in China, with special reference to live transportation. Yang, Guoliang,Chen, Xuefeng,Wang, Junyi,Gao, Qiang,Frinsko, Mike,Hu, Guo. 2012

[9]Characterization of a novel nm23 gene and its potential roles in gametogenesis in the prawn Macrobrachium rosenbergii (de Man, 1879) (Crustacea: Decapoda). Song, Ya-Nan,Lu, Cui-Yun,Chen, Jie,Qiu, Gao-Feng,Lu, Cui-Yun.

[10]Three different anti-lipopolysaccharide factors identified from giant freshwater prawn, Macrobrachium rosenbergii. Ren, Qian,Jie-Du,Hui, Kai-Min,Wang, Wen,Ren, Qian,Jie-Du,Hui, Kai-Min,Wang, Wen,Zhang, Zhao,Zhang, Chi-Yu,Li, Xin-chang. 2012

[11]Effects of anthraquinones extracted from Rheum officinale Bail on the growth, non-specific immune response of Macrobrachium rosenbergii. Liu, Bo,Ge, Xianping,Xie, Jun,Xu, Pao,Liu, Bo,Ge, Xianping,He, Yanhui,Xie, Jun,Xu, Pao,He, Yijin,Zhou, Qunlan,Pan, Liangkun,Chen, Ruli. 2010

[12]Identification and function analysis of ras-related nuclear protein from Macrobrachium rosenbergii involved in Spiroplasma eriocheiris infection. Ning, Mingxiao,Xiu, Yunji,Yuan, Meijun,Bi, Jingxiu,Liu, Min,Wei, Panpan,Yan, Yuye,Gu, Wei,Wang, Wen,Meng, Qingguo,Xiu, Yunji,Gu, Wei,Meng, Qingguo. 2017

[13]Genetic parameters of survival for six generations in the giant freshwater prawn Macrobrachium rosenbergii. Luan, Sheng,Luo, Kun,Kong, Jie,Wang, Junyi,Yang, Guoliang,Chen, Xuefeng,Gao, Qiang,Wang, Junyi,Yang, Guoliang,Chen, Xuefeng,Gao, Qiang,Hu, Honglang.

[14]iTRAQ-based quantitative proteomic analysis of Macrobrachium rosenbergii hemocytes during Spiroplasma eriocheiris infection. Hou, Libo,Xiu, Yunji,Wang, Jian,Liu, Yuhan,Gu, Wei,Wang, Wen,Meng, Qingguo,Xiu, Yunji,Liu, Xiaoqian,Hou, Libo,Xiu, Yunji,Wang, Jian,Liu, Yuhan,Gu, Wei,Wang, Wen,Meng, Qingguo.

[15]The effects of copper, iron and zinc on digestive enzyme activity in the hybrid tilapia Oreochromis niloticus (L.) x Oreochromis aureus (Steindachner). Li, J. -S.,Li, J. -L.,Wu, T. -T.. 2007

[16]Dietary copper affects antioxidant and immune activity in hybrid tilapia (Oreochromis niloticusxOreochromis aureus). Tang, L.,Huang, K.,Sun, L.,Huang, Q.,Bi, Y.,Xie, J.,Yu, D.. 2017

[17]Preparation and antioxidant properties of tilapia (Oreochromis niloticus) protein hydrolysates-copper complex. Hu, Xiao,Li, Laihao,Wu, Yanyan,Yang, Xianqing,Lin, Wanling,Chen, Shengjun,Hao, Shuxian,Ma, Sairui. 2013

[18]Microbial Activities' Influence on Three Kinds of Metal Material Corrosion Behaviors. Li, Xia,Chen, Haiyan,Li, Huanyuan,Chen, Pimao,Qing, Chuangxing. 2017

[19]Copper Acute Toxicity Tests with Schizothorax o'connori Lloyd and Schizothorax waltoni Regan: A Biomonitor of Heavy Metal Pollution in a High Altitude Area?. Shao, Jian,Xie, Congxin,Qin, Jianhui,Huo, Bin,Luo, Yangzhi,Shao, Jian.

[20]The valve movement response of three freshwater mussels Corbicula fluminea Muller 1774, Hyriopsis cumingii Lea 1852, and Anodonta woodiana Lea 1834 exposed to copper. Liu, Hongbo,Chen, Xiubao,Yang, Jian,Kang, Ik Joon,Shimasaki, Yohei,Oshima, Yuji.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台