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PuERF008-PuFAD2 module regulates aroma formation via the fatty acid pathway in response to calcium signaling in 'Nanguo' pear

文献类型： 外文期刊

作者： Liu, Zhi 1 ; Qin, Gai-Hua 2 ; Wei, Shu-Wei 3 ; Yan, Long-Fei 1 ; Ning, Zhi-Yuan 1 ; Wang, Hong 4 ; Cao, Zhen 2 ; Hu, Kang-Di 1 ; Yao, Gai-Fang 1 ; Zhang, Hua 1 ;

作者机构： 1.Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Anhui, Peoples R China

2.Minist Agr & Rural Affairs, Anhui Acad Agr Sci, Inst Hort Res, Key Lab Germplasm Innovat & Utilizat Hort Crop Coc, Hefei 230031, Peoples R China

3.Shandong Inst Pomol, Tai An 271000, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Pomol, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

关键词： 'Nanguo' pear; Aroma; Unsaturated fatty acid metabolic pathway; Calcium signaling; PuERF008-PuFAD2

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2024 年 214 卷

页码：

收录情况： SCI

摘要： Calcium acts as a secondary messenger in plants and is essential for plant growth and development. However, studies on the pathway of aroma synthesis in 'Nanguo' pear (Pyrus ussriensis Maxim.) are scarce. In this study, a bioinformatics analysis of transcriptomic data from calcium-treated 'Nanguo' pear was performed, which identified two fatty acid desaturases, PuFAD2 and PuFAD3, and eight AP2/ERF transcription factors, all exhibiting the same expression patterns. Transient expression experiments showed overexpression of PuFAD2 and PuFAD3 significantly increased the levels of aromatic substrates linoleic acid, hexanal, linolenic acid, and (E)-2hexenal, but RNAi (RNA interference) had the opposite expression. Promoter sequences analysis revealed that PuFAD2 and PuFAD3 have ERE (estrogen response element) motifs on their promoters. The strongest activation of PuFAD2 by PuERF008 was verified using a dual-luciferase reporting system. Additionally, yeast one-hybrid and electrophoretic mobility shift assays revealed PuERF008 could active PuFAD2. Transient overexpression and RNAi analyses of PuERF008 showed a strong correlation with the expression of PuFAD2. This study provides insights into the process of aroma biosynthesis in 'Nanguo' pear and offers a theoretical basis for elucidating the role of calcium signaling in aroma synthesis.

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