您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage

文献类型： 外文期刊

作者： Qiu, Lihua 1 ; Jiang, Shigui 1 ; Zhou, Falin 1 ; Zhang, Dianchang 1 ; Huang, Jianhua 1 ; Guo, Yihui 1 ;

作者机构： 1.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Biotechnol & Aquiculture Lab, Guangzhou 510300, Guangdong, Peoples R China

关键词： cloning;elongation factor 2;expression;black tiger shrimp (Penaeus monodon)

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2008 年 35 卷 3 期

页码：

收录情况： SCI

摘要： The techniques of homology cloning and anchored PCR were used to clone the elongation factor 2 (EF-2) gene from black tiger shrimp (Penaeus monodon). The full length cDNA of black tiger shrimp EF-2 (btsEF-2) contained a 5' untranslated region (UTR) of 73 bp, an ORF of 2541 bp encoding a polypeptide of 846 amino acids with an estimated molecular mass of 95 kDa, and a 3( UTR of 112 bp. The searches for protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of btsEF-2 was homological to the EF-2 of other species and even the mammalians. The conserved signature sequence of EF-2 gene family, GTPase effector domain and ADP-ribosylation domain were found in the btsEF-2 deduced amino acid sequence. The temporal expressions of gene in the different ovarian stages were measured by real time PCR. The mRNA expressions of the gene were constitutively expressed in ovary and different during the maturation stages. The result indicated that EF-2 gene was constitutively expressed and could play a critical role in the ovarian maturation stage.

  • 相关文献

[1]Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon). Wang, Y.,Fu, M. J.,Zhao, C.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Wang, Y.,Fu, M. J.,Zhao, C.,Bao, W. Y.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Yang, Q. B.. 2016

[2]Molecular cloning and mRNA expression of peroxiredoxin gene in black tiger shrimp (Penaeus monodon). Qiu, Lihua,Jiang, Shigui,Wang, Weifang,Zhou, Falin,Huang, Jianhua,Li, Jianzhu,Yang, Qibin,Ma, Zhuojun.

[3]Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus. Zhang, Yu-Xi,Zhang, Bo,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng. 2012

[4]Na+/K+-ATPase alpha-subunit in swimming crab Portunus trituberculatus: molecular cloning, characterization, and expression under low salinity stress. Han, Xiaolin,Liu, Ping,Gao, Baoquan,Wang, Haofeng,Duan, Yafei,Xu, Wenfei,Chen, Ping,Han, Xiaolin. 2015

[5]Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress. Ren Hai,Wu Jianhua,Ren Hai,Li Jian,Li Jitao,Liu Ping,Liang Zhongxiu. 2015

[6]Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress. Ren Hai,Ren Hai,Li Jian,Li Jitao,Ying Yu,Ge Hongxing,Li Dongli,Yu Tianji. 2015

[7]Molecular cloning, characterization and expression analysis of multiple leptin genes in Jian carp (Cyprinus carpio var. Jian). Tang, Yongkai,Yu, Juhua,Xu, Pao,Tang, Yongkai,Yu, Juhua,Li, Hongxia,Xu, Pao,Li, Jianlin,Ren, Hongtao. 2013

[8]Identification, cloning and expression analysis of an alpha-CGTase produced by stain Y112. Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Huang, Li-Ping,Chen, Xiao-tong. 2017

[9]Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus). Qiu, Lihua,Yang, Keng,Zhang, Hanhua,Li, Jianzhu,Zou, Falin,Jiang, Shigui,Lin, Liansheng. 2011

[10]Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Liu, Bo,He, Yijin,Cheng, Yanfen,Zhou, Qunlan,Pan, Liangkun,Ming, Jianhua,Liu, Wenbin. 2010

[11]Cloning of a heat shock protein 90 (HSP90) gene and expression analysis in the ridgetail white prawn Exopalaemon carinicauda. Li, Jitao,Han, Junying,Chen, Ping,Chang, Zhigjang,He, Yuying,Liu, Ping,Wang, Qingyin,Li, Jian. 2012

[12]Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunus trituberculatus. Wang, Qingyin. 2010

[13]Molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab Portunus trituberculatus. Li, Jitao,Chen, Ping,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[14]cDNA cloning, tissues, embryos and larvae expression analysis of Sox10 in half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Deng, Si-Ping,Chen, Song-Lin,Deng, Si-Ping.

[15]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[16]Molecular cloning and sexually dimorphic expression of DMRT4 gene in Oreochromis aureus. Gan, Xi,Luo, Yongju,Wu, Tingting,Cao, Jinling,Chen, Jianjie.

[17]Molecular cloning and expression analysis of cytochrome P450 3A gene in the turbot Scophthalmus maximus. Sun, Airong,Li, Jian,Chang, Zhiqiang,Li, Jitao,Wang, Qi,Sun, Airong,Huang, Jingzhou,Wang, Qi.

[18]Molecular cloning and mRNA expression of M-phase phosphoprotein 6 gene in black tiger shrimp (Penaeus monodon). Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang,Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang.

[19]Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis). Chen, Song-lin,Xu, Tian-jun. 2011

[20]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台