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GmWAK1, Novel Wall-Associated Protein Kinase, Positively Regulates Response of Soybean to Phytophthora sojae Infection

文献类型： 外文期刊

作者： Zhao, Ming 1 ; Li, Ninghui 1 ; Chen, Simei 1 ; Wu, Junjiang 2 ; He, Shengfu 1 ; Zhao, Yuxin 1 ; Wang, Xiran 1 ; Chen, Xiaoyu 1 ; Zhang, Chuanzhong 1 ; Fang, Xin 1 ; Sun, Yan 1 ; Song, Bo 1 ; Liu, Shanshan 1 ; Liu, Yaguang 1 ; Xu, Pengfei 1 ; Zhang, Shuzhen 1 ;

作者机构： 1.Northeast Agr Univ, Soybean Res Inst, Chinese Educ Minist, Key Lab Soybean Biol, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Soybean Res Inst, Key Lab Soybean Cultivat, Minist Agr, Harbin 150030, Peoples R China

3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150030, Peoples R China

关键词： Glycine max; wall-associated protein kinase; annexin; pathogenesis-related genes; SA; ROS

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Phytophthora root rot is a destructive soybean disease worldwide, which is caused by the oomycete pathogen Phytophthora sojae (P. sojae). Wall-associated protein kinase (WAK) genes, a family of the receptor-like protein kinase (RLK) genes, play important roles in the plant signaling pathways that regulate stress responses and pathogen resistance. In our study, we found a putative Glycine max wall-associated protein kinase, GmWAK1, which we identified by soybean GmLHP1 RNA-sequencing. The expression of GmWAK1 was significantly increased by P. sojae and salicylic acid (SA). Overexpression of GmWAK1 in soybean significantly improved resistance to P. sojae, and the levels of phenylalanine ammonia-lyase (PAL), SA, and SA-biosynthesis-related genes were markedly higher than in the wild-type (WT) soybean. The activities of enzymatic superoxide dismutase (SOD) and peroxidase (POD) antioxidants in GmWAK1-overexpressing (OE) plants were significantly higher than those in in WT plants treated with P. sojae; reactive oxygen species (ROS) and hydrogen peroxide (H2O2) accumulation was considerably lower in GmWAK1-OE after P. sojae infection. GmWAK1 interacted with annexin-like protein RJ, GmANNRJ4, which improved resistance to P. sojae and increased intracellular free-calcium accumulation. In GmANNRJ4-OE transgenic soybean, the calmodulin-dependent kinase gene GmMPK6 and several pathogenesis-related (PR) genes were constitutively activated. Collectively, these results indicated that GmWAK1 interacts with GmANNRJ4, and GmWAK1 plays a positive role in soybean resistance to P. sojae via a process that might be dependent on SA and involved in alleviating damage caused by oxidative stress.

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