Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
文献类型： 外文期刊
作者： Peng, Junbo 1 ; Wu, Linna 1 ; Zhang, Wei 1 ; Zhang, Qi 1 ; Xing, Qikai 1 ; Wang, Xuncheng 1 ; Li, Xinghong 1 ; Yan, Jiye 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing, Peoples R China
关键词： Lasiodiplodia theobromae; CFEM domain; cell death; immune response; temperature
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in Lasiodiplodia theobromae and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system. The transcriptional levels of most CFEM genes, except for LtCFEM2 and LtCFEM6, were significantly elevated during infection. In addition, almost all LtCFEM genes, apart from LtCFEM2, LtCFEM3, and LtCFEM6, were transcriptionally up-regulated at 35 degrees C in contrast to that at 25 degrees C and 30 degrees C. As two elicitors, LtCFEM1 induced local yellowish phenotype and LtCFEM4 triggered cell death in Nicotiana benthamiana leaves. Furthermore, these proteins displayed distinct subcellular localizations when expressed transiently in N. benthamiana. Moreover, two genes, LtCFEM7 and LtCFEM8, were found to be spliced alternatively by RT-PCR and sequencing. Therefore, our data suggest that LtCFEM proteins play important roles in multiple aspects, including pathogenicity and plant immune response, which will enhance our understanding of the sophisticated pathogenic mechanisms of plant opportunistic pathogen L. theobromae.
- 相关文献
作者其他论文 更多>>
-
The GmSTF1/2-GmBBX4 negative feedback loop acts downstream of blue-light photoreceptors to regulate isoflavonoid biosynthesis in soybean
作者：Song, Zhaoqing;Zhao, Fengyue;Chu, Li;Lin, Huan;Xiao, Yuntao;Fang, Zheng;Yu, Deyue;Gai, Junyi;Xu, Dongqing;Wang, Xuncheng;Dong, Jie;Lyu, Xiangguang;Liu, Bin
关键词：photoreceptor; light signaling; isoflavonoid; GmSTF; GmBBX4; soybean
-
Prediction of maize cultivar yield based on machine learning algorithms for precise promotion and planting
作者：Han, Yanyun;Wang, Kaiyi;Yang, Feng;Pan, Shouhui;Liu, Zhongqiang;Zhang, Qiusi;Zhang, Qi;Han, Yanyun;Wang, Kaiyi;Yang, Feng;Pan, Shouhui;Liu, Zhongqiang;Zhang, Qiusi;Zhang, Qi
关键词：Prediction of maize cultivar yield; Machine learning; Random forest; Levenberg - Marquardt neural network; Multilayer perceptron neural network; Assessment of varieties
-
Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A
作者：Xu, Miqi;Wang, Yi-Yuan;Wu, Yujie;Shan, Ziyan;Tao, Kunying;Qian, Kaiqiang;Wu, Qingqing;Ling, Jun-Jie;Xu, Miqi;Wang, Yi-Yuan;Wu, Yujie;Shan, Ziyan;Tao, Kunying;Qian, Kaiqiang;Ling, Jun-Jie;Zhou, Xiuhong;Wang, Xuncheng;Li, Jian;Deng, Xing Wang;Deng, Xing Wang;Deng, Xing Wang
关键词：cotyledon development; green light; photobody; phytochrome; PIF3
-
Bark transcriptome analyses reveals molecular mechanisms involved in tapping panel dryness occurrence and development in rubber tree (Hevea brasiliensis)
作者：Yue, Yifan;Deng, Zhi;Wang, Difei;Li, Dejun;Wang, Xuncheng;Xia, Zhihui;Li, Yao;Yin, Han;Yue, Yifan;Wang, Difei;Li, Dejun
关键词：Tapping panel dryness (TPD); Hevea brasiliensis; Molecular mechanisms; Transcriptome analyses; TPD-related genes
-
Developing a comprehensive evaluation model of variety adaptability based on machine learning method
作者：Han, Yanyun;Wang, Kaiyi;Zhang, Qi;Yang, Feng;Pan, Shouhui;Liu, Zhongqiang;Zhang, Qiusi;Han, Yanyun;Wang, Kaiyi;Zhang, Qi;Yang, Feng;Pan, Shouhui;Liu, Zhongqiang;Zhang, Qiusi
关键词：Maize variety adaptability evaluation; Variety adaptability comprehensive evaluation index; Entropy weight method; Machine learning method
-
Microfungi Associated with Peach Branch Diseases in China
作者：Zhou, Ying;Li, Shifang;Zhou, Ying;He, Zhizheng;Zhang, Wei;Liu, Mei;Song, Jinyan;Yan, Jiye;Zhou, Ying;Fan, Zaifeng;Manawasinghe, Ishara S.
关键词：diversity; peach diseases; morphology; phylogenetic analyses; new species; new records
-
TrG2P: A transfer-learning-based tool integrating multi-trait data for accurate prediction of crop yield
作者：Li, Jinlong;Zhang, Dongfeng;Yang, Feng;Zhang, Qiusi;Pan, Shouhui;Zhao, Xiangyu;Zhang, Qi;Han, Yanyun;Wang, Kaiyi;Zhao, Chunjiang;Li, Jinlong;Zhang, Dongfeng;Yang, Feng;Zhang, Qiusi;Pan, Shouhui;Zhao, Xiangyu;Zhang, Qi;Han, Yanyun;Wang, Kaiyi;Zhao, Chunjiang;Yang, Jinliang;Yang, Jinliang
关键词：crop; genotype to phenotype; transfer learning; yield prediction; multi-trait
