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SNP-based high-density genetic map construction and candidate gene identification for fruit quality traits of Dimocarpus longan Lour

文献类型： 外文期刊

作者： Jue, Dengwei 1 ; Liu, Liqin 2 ; Sang, Xuelian 1 ; Shu, Bo 3 ; Wang, Jiahui 2 ; Wang, Yicheng 2 ; Zhang, Chenchen 1 ; Shi, 1 ;

作者机构： 1.Chongqing Univ Arts & Sci, Chongqing Engn Res Ctr Special Plant Seedling, Collaborat Innovat Ctr Special Plant Ind Chongqin, Inst Special Plants,Chongqing Key Lab Econ Plant, Yongchuan 402160, Peoples R China

2.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang 524091, Peoples R China

3.Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Peoples R China

关键词： Fruit breeding; Longan; Genetic map; Stable QTL; Candidate genes

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2021 年 284 卷

页码：

收录情况： SCI

摘要： Longan is an economically important fruit tree that is cultivated in subtropical regions worldwide. For longan breeding, traits associated with yield and quality are considered desirable. Thus, fine mapping of quantitative trait loci (QTLs) and mining candidate genes for longan fruit yield and quality would benefit longan breeding. In this study, we constructed the first single-nucleotide polymorphism (SNP)-based genetic map of an F-1 longan population using restriction site-associated DNA sequencing. A total of 1,464,790,436 clean reads were generated from 200 F-1 progeny from 'Fengliduo' (female) x 'Dawuyuan' (male) and their parents. The overall integrated map contained 8014 SNP markers, which were assigned to 15 linkage groups with a total length of 2873.39 cM, an average distance of 0.36 cM, and 99.96 % of gaps having a genetic distance of <5 cM. Based on two successive years of fruit phenotyping and this high-density linkage map, 17 stable QTLs were identified, including 12 for single fruit weight (SFW) and 5 for edible rate, but no stable QTLs were identified for soluble solid content. Finally, three key candidate genes, namely, Dlo_007693.1 (DlEXP4), Dlo_011045.1 (DlCNR8), and dlo_034894.1 (DlCYP450), for SFW were identified based on RNA sequencing data and quantitative reverse-transcription polymerase chain reaction. In conclusion, this is the first study on the construction of a high-density genetic map of longan using next-generation sequencing based on the largest mapping population. These results provide valuable information for marker-assisted selection breeding and mining of candidate genes related to fruit traits in longan.

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