PpERF-CRF3 selected by transcriptomic analysis plays key roles in the regulation of ABA alleviating chilling injury in peach fruit
文献类型： 外文期刊
作者： Yang, Wenteng 1 ; He, Yuan 1 ; Li, Wenhui 1 ; Dai, Mei 1 ; Wu, Bin 2 ; Zhang, Zheng 2 ; Shi, Jingying 1 ; Song, Zunyang 1 ;
作者机构： 1.Shandong Agr Univ, Coll Food Sci & Engn, Key Lab Food Proc Technol & Qual Control Shandong, Tai An, Peoples R China
2.Inst Agroprod Storage & Proc, Xinjiang Acad Agr Sci, Urumqi, Xinjiang, Peoples R China
3.Xinjiang Acad Agr Sci, Xinjiang Key Lab Proc & Preservat Agr Prod, Urumqi, Xinjiang, Peoples R China
关键词： Peach fruit; Abscisic acid; Chilling injury; Transcriptome analysis; Proline synthesis; ERF
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 282 卷
页码：
收录情况： SCI
摘要： Abscisic acid (ABA) is widely utilized to mitigate chilling injury (CI) of fruit. However, the molecular mechanism of ABA alleviates CI in peach fruit remain unclear. Herein, 10(-4) M ABA treatment significantly mitigated the CI of peach fruit by reducing relative conductivity and malondialdehyde content, while increasing proline and endogenous ABA content. Transcriptomic analysis indicated that an abundant number of differentially expressed genes were altered by ABA treatment, which primarily enriched pathways such as plant hormone signal transduction, glycerophospholipid metabolism and phenylpropanoid biosynthesis. RNA-Seq results indicate that ABA modulates the transcription of genes involved in auxin, ABA and ethylene signal transduction, as well as in cell wall degradation, antioxidant, fatty acid desaturation and proline metabolism. RT-qPCR confirmed the RNA-Seq results, ABA treatment induced the transcription of proline metabolism related genes (PpP5CR2, PpP5CS, PpP5CS1) and PpERF-CRF3. Particularly noteworthy, as a nuclear protein, PpERF-CRF3 activated the expression of PpP5CR2 and PpP5CS by directly binding to their promoters and over-expression PpERF-CRF3 increased proline content and enhanced PpP5CR2 and PpP5CS expression. Overall, these findings suggest that ABA mitigates CI in peach fruit may be by mediating these pathways, and PpERF-CRF3 potentially involves this process by stimulating the expression of genes related to proline synthesis.
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