文献类型： 外文期刊

作者： Han, Mingming ³ ; Liang, Ji ³ ; Wang, Kai ⁴ ; Si, Qin ⁹ ; Zhu, Chenxi ³ ; Zhao, Yunlong ⁵ ; Khan, Nurzalina Abdul Karim ³ ; Abdullah, Anisah Lee Binti ³ ; Shau-Hwai, Aileen Tan ³ ; Li, Yi Ming ⁶ ; Zhou, Zihan ³ ; Jiang, Chunqi ⁷ ; Liao, Jiayuan ⁸ ; Tay, Yi Juin ³ ; Qin, Wei ¹ ; Jiang, Qichen ² ;

作者机构： 1.Nanjing Med Univ, Nanjing Hosp 1, Dept Cardiothorac Surg, Nanjing 210017, Peoples R China

2.Freshwater Fisheries Res Inst Jiangsu Prov, 79 Chating East St, Nanjing 210017, Peoples R China

3.Univ Sains Malaysia, Minden 11800, Penang, Malaysia

4.Chinese Acad Med Sci, Natl Ctr Resp Med, State Key Lab Resp Hlth & Multimorbid, Natl Clin Res Ctr Resp Dis,Inst Resp Med,Dept Pulm, Beijing, Peoples R China

5.East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China

6.Chinese Acad Fishery Sci, Fishery Machinery & Instrument Res Inst, Beijing, Peoples R China

7.Univ Tokyo, Atmosphere & Ocean Res Inst, Chiba 2778564, Japan

8.Sun Yat Sen Univ, Sch Atmospher Sci, Southern Marine Sci & Engn Guangdong Lab, Zhuhai 519082, Peoples R China

9.Jiangsu Maritime Inst, 309 Gezhi Rd, Nanjing 211100, Jiangsu, Peoples R China

关键词： Nano-plastics; Human lung diseases; ROS; Calcium channel

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：8.0； 五年影响因子：8.7 ）

ISSN： 0048-9697

年卷期： 2024 年 953 卷

页码：

收录情况： SCI

摘要： The extensive use of plastic products has exacerbated micro/nanoplastic (MPs/NPs) pollution in the atmosphere, increasing the incidence of respiratory diseases and lung cancer. This study investigates the uptake and cytotoxicity mechanisms of polystyrene (PS) NPs in human lung epithelial cells. Transcriptional analysis revealed significant changes in cell adhesion pathways following PS-NPs exposure. Integrin alpha 5 beta 1-mediated beta 1-mediated endocytosis was identified as a key promoter of PS-NPs entry into lung epithelial cells. Overexpression of integrin alpha 5 beta 1 enhanced PS-NPs internalization, exacerbating mitochondrial Ca2+ dysfunction and depolarization, which induced reactive oxygen species (ROS) production. Mitochondrial dysfunction triggered by PS-NPs led to oxidative damage, inflammation, DNA damage, and necrosis, contributing to lung diseases. This study elucidates the molecular mechanism by which integrin alpha 5 beta 1 facilitates PS-NPs internalization and enhances its cytotoxicity, offering new insights into potential therapeutic targets for microplastic-induced lung diseases.