Ratio of carbon and nitrogen in fertilizer treatment drives distinct rhizosphere microbial community composition and co-occurrence networks
文献类型： 外文期刊
作者： Zhu, Ruifen 1 ; Liu, Chang 2 ; Xu, Yuan Dong 2 ; He, Wei 2 ; Liu, Jielin 3 ; Chen, Jishan 2 ; An, Yajun 4 ; Shi, Shangli 1 ;
作者机构： 1.Gansu Agr Univ, Pratacultural Coll, Lanzhou, Peoples R China
2.Chongqing Acad Anim Sci, Pratacultural Inst, Rongchang, Peoples R China
3.Heilongjiang Acad Agr Sci, Pratacultural Inst Sci, Harbin, Peoples R China
4.Gansu Yasheng Agr Res Inst Co Ltd, Lanzhou, Peoples R China
关键词： ratio of carbon and nitrogen; rhizosphere; microbial community composition; co-occurrence networks; alfalfa
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Fertilization is the main strategy to accelerate vegetation restoration and improve the rhizosphere microbial community in the northeast China. However, the responses of rhizosphere microbial community structure, specific microbial community and symbiotic pattern to manure fertilization in grassland (alfalfa only) are not well clear. In this study, the variation of bacterial community structures in R_Manure (extracted liquid of fermented cow manure), E_Manure (extracted residue of fermented cow manure), F_Manure (full fermented cow manure), and Control (without fermented cow manure) collected from the rhizosphere microbial community of alfalfa were analyzed by the application of an Illumina HiSeq high-throughput sequencing technique. A total of 62,862 microbial operational taxonomic units (OTUs) were detected and derived from 21 phyla of known bacteria. The dominant bacteria in the rhizosphere include Proteobacteria (70.20%), Acidobacteria (1.24%), Actinobacteria (2.11%), Bacteroidetes (6.15%), Firmicutes (4.21%), and Chlorofexi (2.13%) accounting for 86% of the dominant phyla in all treatments. At the genus level, the dominant genus include NB1-j, Lysobacter, Alphaproteobacteria, Subgroup_6, Actinomarinales, Saccharimonadales, Aneurinibacillus, MO-CFX2, SBR1031, Caldilineaceae, and so on with the average relative abundance (RA) of 1.76%, 1.52%, 1.30%, 1.24%, 1.61%, 2.39%, 1.36%, 1.42%, 1.27%, and 1.03%, respectively. Bacterial diversities and community structures were significantly differentiated by different treatments of fertilization. The results of community structure composition showed that R_Manure treatment significantly increased the population abundance of Firmicutes, Chlorofexi, and Patescibacteria by 34.32%, 6.85%, and 2.70%, and decreased the population abundance of Proteobacteria and Actinobacteria by 16.83% and 1.04%, respectively. In addition, it showed that all treatments significantly resulted in an increase or decrease at the genus level. R_Manure had the higher richness and diversity of the bacterial community, with the greatest topology attributes of the co-occurrence networks. Through the analysis of the molecular ecological network (MENA), the co-occurrence networks had a shorter average path distance and diameter in R_Manure than in others, implying more stability to environmental changes. Redundancy analysis (RDA) showed that the ratio of carbon and nitrogen (C/N) was the main factor affecting rhizosphere microbial community composition while driving distinct rhizosphere bacterial community and its co-occurrence networks. The R_Manure associated with more C/N had relatively complex microbial co-occurrence network with a large number of nodes and edges, while the microbial network of others associated with less C/N had fewer taxa with loose mutual interactions. These results suggested that organic fertilizer with high C/N can regulate the rhizosphere microorganism, while high C/N can determine bacterial community structures, specific bacterial taxa, and their relationships with the nodule size of alfalfa. These significant changes can be used to evaluate soil fertility and fertilizer management in the artificial grassland system, while the potential biological indicators of the rhizosphere microbial community will play an important role in future eco-agriculture.
- 相关文献
作者其他论文 更多>>
-
Extreme drought impacts have been underestimated in grasslands and shrublands globally
作者：Smith, Melinda D.;Knapp, Alan K.;Ohlert, Timothy;Smith, Melinda D.;Knapp, Alan K.;Wilkins, Kate D.;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Wilfahrt, Peter;Anderson, Maggie;Borer, Elizabeth T.;Isbell, Forest;Seabloom, Eric W.;Collins, Scott L.;Sala, Osvaldo E.;Jordan, Samuel;Dukes, Jeffrey S.;Phillips, Richard P.;Beier, Claus;Yahdjian, Laura;Biancari, Lucio;Bondaruk, Viviana Florencia;Onatibia, Gaston R.;Pineiro-Guerra, Juan Manuel;Gherardi, Laureano A.;Larsen, Klaus Steenberg;Fraser, Lauchlan H.;Jentsch, Anke;Niu, Yujie;Loik, Michael E.;Maestre, Fernando T.;Valdecantos, Alejandro;Maestre, Fernando T.;Gozalo, Beatriz;Maturano-Ruiz, Adrian;Valdecantos, Alejandro;Power, Sally A.;Chieppa, Jeff;Nielsen, Uffe N.;Yu, Qiang;Felton, Andrew J.;Munson, Seth M.;Luo, Yiqi;Bauerle, Taryn;Abdoli, Hamed;Abedi, Mehdi;Bahalkeh, Khadijeh;Alados, Concepcion L.;Castillo-Garcia, Miguel;Pueyo, Yolanda;Alberti, Juan;Bruschetti, Carlos Martin;Cardoni, Augusto;Daleo, Pedro;Iribarne, Oscar;Alon, Moshe;Cohen, Ofer;Sternberg, Marcelo;An, Hui;Anacker, Brian;Auge, Harald;Auge, Harald;Eisenhauer, Nico;Hoss, Daniela;Roscher, Christiane;Sunnemann, Marie;Bachle, Seton;Bachle, Seton;Bahn, Michael;Ingrisch, Johannes;Batbaatar, Amgaa;Cahill, James F., Jr.;Batbaatar, Amgaa;Bork, Edward W.;Carlyle, Cameron N.;Behn, Kai;Beil, Ilka;Kreyling, Juergen;Malyshev, Andrey V.;Blindow, Irmgard;Dahlke, Sven;Byrne, Kerry M.;Calvo, Dianela A.;Leder, Cintia V.;Peter, Guadalupe;Carbognani, Michele;Forte, T'ai G. W.;Petraglia, Alessandro;Chang, Scott X.;Cianciaruso, Marcus V.;Guidoni-Martins, Karlo G.;Nobre, Paola;Cordeiro, Amanda L.;Cusack, Daniela F.;Dietterich, Lee H.;D'Antonio, Carla M.;Dietterich, Lee H.;Doherty, Tim S.;Greenville, Aaron C.;Wardle, Glenda M.;Dubbert, Maren;Ebeling, Anne;Eisenhauer, Nico;Fischer, Felicia M.;Hoss, Daniela;Sunnemann, Marie;Hoss, Daniela;Pillar, Valerio D.;Fischer, Felicia M.;Gebauer, Tobias;Anacker, Brian;Haugum, Siri Vatso;Vandvik, Vigdis;Hautier, Yann;Hefting, Mariet;Henry, Hugh A. L.;Johnson, Yari;Kelly, Eugene F.;Kimmel, Kaitlin;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kubert, Angelika;Werner, Christiane;Lamb, Eric G.;Larson, Julie;O'Connor, Rory C.;Lawson, Jason;Plowes, Robert M.;Linstadter, Anja;Orban, Ildiko;Liu, Jielin;Liu, Shirong;Longo, Grisel;Loydi, Alejandro;Rodriguez, Dana Aylen;Scarfo, Maria Cecilia;Luan, Junwei;Wang, Yi;Luan, Junwei;Wang, Yi;Lubbe, Frederick Curtis;Wiehl, Georg;Mackie-Haas, Kathleen;Stampfi, Andreas;Merchant, Thomas;Metcalfe, Daniel B.;Mori, Akira S.;Mori, Akira S.;Mudongo, Edwin;Newman, Gregory S.;Souza, Lara;Nimmo, Dale;Ogaya, Roma;Penuelas, Josep;Osborne, Brooke;Otfinowski, Rafael;Partel, Meelis;Pineiro-Guerra, Juan Manuel;Peri, Pablo L.;Picon-Cochard, Catherine;Pineiro-Guerra, Juan Manuel;Ploughe, Laura W.;Portales-Reyes, Cristy;Reed, Sasha C.;Ritchie, Euan G.;Roscher, Christiane;Sanchez, Ana M.;Santo, Braulio A.;Shi, Baoku;Sun, Wei;Souza, Lara;Standish, Rachel J.;Zeiter, Michaela;Stampfi, Andreas;Zeiter, Michaela;Standish, Rachel J.;Tedder, Michelle;Thorvaldsen, Pal;Tian, Dashuan;Zhu, Juntao;Zong, Ning;Tielborger, Katja;van den Brink, Liesbeth;Vankoughnett, Mathew R.;Velle, Liv Guri;Wang, Changhui;Wei, Cunzheng;Williams, Jennifer L.;Wolf, Amelia A.;Zhang, Fawei;Zuo, Xiaoan;Zuo, Xiaoan;Gebauer, Tobias;Ploughe, Laura W.
关键词：climate extreme; Drought-Net; International Drought Experiment; productivity
-
Metagenomics study of soil microorganisms involved in the carbon cycle in a saline-alkaline meadow steppe in the Songnen Plain in Northeast China
作者：Xiao, Huichuan;Wei, Yinzhu;Sun, Xuetong;Song, Xue;Bai, Zhenjian;Hu, Guofu;Qin, Ligang;Liu, Jielin
关键词：saline-alkaline meadow steppe; soil microorganisms; carbon cycle immobilization-related functional genes; structural equation model; soil carbon cycle
-
Generation of New β-Conglycinin-Deficient Soybean Lines by Editing the lincRNA lincCG1 Using the CRISPR/Cas9 System
作者：Song, Bo;Luo, Tingting;Fan, Yuanhang;Qiu, Zhendong;Tian, Yusu;Shang, Yuzhuo;Ma, Chongxuan;Liu, Chang;Cao, Qingqian;Peng, Yuhan;Xu, Pengfei;Zhang, Shuzhen;Liu, Shanshan;Song, Bo;Li, Ming;Krishnan, Hari B.;Krishnan, Hari B.;Wang, Zhenhui
关键词：soybean; lincRNA; loss of function; beta-conglycinin deficiency; Cas9-free line
-
CRISPR-Cas9-mediated editing of GmARM improves resistance to multiple stresses in soybean
作者：Luo, Tingting;Ma, Chongxuan;Fan, Yuanhang;Qiu, Zhendong;Tian, Yusu;Shang, Yuzhuo;Liu, Chang;Cao, Qingqian;Peng, Yuhan;Zhang, Shuzhen;Liu, Shanshan;Song, Bo;Li, Ming;Song, Bo
关键词：Soybean; Stress resistance; GmARM; CRISPR/Cas9
-
Factors controlling the contributions of bacterial and fungal residue carbon to soil organic carbon in grassland ecosystems
作者：Yu, Yingchao;Pan, Junxiao;Zhu, Juntao;Zong, Ning;Liu, Shuang;Zhang, Xinyu;Yu, Yingchao;Zhu, Juntao;Liu, Shuang;Zhang, Xinyu;Wu, Honghui;Wu, Honghui;An, Hui;Wang, Changhui;Diao, Huajie;Zuo, Xiaoan;Wei, Cunzheng;Zhang, Fawei;Liu, Jielin;Zhang, Bo;Yu, Qiang;Yu, Qiang
关键词：Fungal residue carbon; Bacterial residue carbon; Extracellular enzyme activity; Soil C/N; Alpine grassland; Temperate grassland
-
A comparison of the mineral element content of 70 different varieties of pear fruit (Pyrus ussuriensis) in China
作者：Liu, Chang;Wu, Yuxia;Ma, Ping;He, Tianming;Liu, Chang;Yu, Wenquan;Li, Honglian;Ren, Aihua;Chen, Guoyou;Ye, Wanjun;Ma, Ping
关键词：Correlation analysis; Mineral element; Peel; Pulp; Pyrus ussuriensis Maxim
-
Soil Anti-Scourabilities of Four Typical Herbaceous Plants and Their Responses to Soil Properties, Root Traits and Slope Position in Northeast China
作者：Wang, Xueshan;Liu, Jielin;Kong, Xiaolei;Peng, Daqing;Zhang, Qiang;Guo, Mingming
关键词：slope erosion; soil anti-scourability; herbaceous plants; root traits; mollisols
