The effects of exogenous additives on the vitality of Chinese pearl oyster ( Pinctada fucata) sperm under different conditions
文献类型： 外文期刊
作者： Li, Minghao 1 ; Fu, Zhengyi 2 ; Yang, Rui 2 ; Yu, Gang 2 ; Zhou, Wenli 1 ; Ma, Zhenhua 2 ;
作者机构： 1.Tianjin Agr Univ, Coll Fisheries, Tianjin, Peoples R China
2.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya, Peoples R China
3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Beijing, Peoples R China
4.Hainan Engn Res Ctr Deep Sea Aquaculture & Proc, Sanya 572018, Peoples R China
5.Int Joint Res Ctr Conservat & Applicat Fishery Res, Sanya 572018, Peoples R China
6.Flinders Univ S Australia, Coll Sci & Engn, Adelaide, Australia
关键词： Chinese pearl oyster; sperm vitality; fructose; glucose; temperature; ammonia water
期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.4； 五年影响因子：0.5 ）
ISSN： 0792-156X
年卷期： 2024 年 76 卷 3 期
页码：
收录情况： SCI
摘要： During the artificial breeding process of Chinese pearl oysters, the quality and vitality of sperm and eggs are pivotal in determining the success rate of fertilization. This study delves into the impact of externally added fructose, glucose, and ammoniated water on sperm vitality and lifespan under varying temperature conditions. The results indicate that the addition of exogenous energy sources and cell protectants, specifically under 4 degrees C conditions, adding 2.8 mmol/L fructose, glucose, and 2 mmol/L ammoniated water to sperm samples significantly enhances sperm vitality and prolongs their lifespan. This provides strong evidence for the feasibility of short-term preservation and vitality enhancement strategies for Chinese pearl oyster sperm and also offers important scientific support for optimizing the artificial reproduction techniques of Chinese pearl oysters.
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