Screening Proteins That Interact With AcHog1 and the Functional Analysis of AcSko1 in Aspergillus cristatus
文献类型： 外文期刊
作者： Shao, Lei 1 ; Liu, Zuoyi 4 ; Liu, Yongxiang 4 ; Tan, Yumei 4 ;
作者机构： 1.Guiyang Healthcare Vocat Univ, Coll Pharm, Guiyang, Guizhou, Peoples R China
2.Guiyang Healthcare Vocat Univ, Guizhou Prov Engn Res Ctr Med Resourceful Healthca, Guiyang, Guizhou, Peoples R China
3.Guizhou Univ, Coll Agr, Guiyang, Guizhou, Peoples R China
4.Guizhou Key Lab Agr Biotechnol, Guiyang, Guizhou, Peoples R China
5.Guizhou Acad Agr Sci, Inst Biotechnol, Guiyang, Guizhou, Peoples R China
6.Zhongkai Univ Agr & Engn, Innovat Inst Plant Hlth, Guangzhou, Guangdong, Peoples R China
关键词： LC-MS/MS; sexual and asexual sporulation; stress response; two hybrid
期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：3.5 ）
ISSN： 0233-111X
年卷期： 2025 年 65 卷 1 期
页码：
收录情况： SCI
摘要： Aspergillus cristatus is a dominant fungus formed during the "flowering" process of Fuzhuan brick tea. Previous research has established that the sporulation of Aspergillus nidulans, a model organism of filamentous fungi, is regulated by light. However, the sporulation of A. cristatus is dependent on osmotic stress. In a previous study, we used pull-down and mass spectrometry to identify proteins that interacted with AcHog1 in A. cristatus when cultured under different conditions of osmotic stress. In the present study, we analyzed the proteins we identified previously to investigate their functional role. The AA1E3BER4 protein was located downstream of Hog1 in the HOG branch pathway and was identified that was regulated by AcHog1. Furthermore, yeast two-hybrid analysis showed that AA1E3BER4 interacted with AcHog1. In addition, we knocked out and complemented the Acsko1 gene encoding the AA1E3BER4 protein. We found that the number of sexual and asexual spores were downregulated by 3.81- and 4.57-fold, respectively, in the Delta Acsko1 strain. The sensitivity of the Delta Acsko1 strain to sorbitol and sucrose, as regulators of osmotic stress, increased, and the sensitivity to high sucrose was higher than that of sorbitol. Acsko1 also regulated the response of A. cristatus to oxidative stress, Congo red, and SDS (sodium dodecyl sulfate). In addition, the deletion of Acsko1 significantly increased the pigment of the Delta Acsko1 strain. This is the first study to report the role of the sko1 gene in oxidative stress, stress-induced damage to the cell wall, and pigment in Aspergillus cristatus.
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