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Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing

文献类型： 外文期刊

作者： Yang, Tianxia 1 ; Ali, Muhammad 1 ; Lin, Lihao 1 ; Li, Ping 3 ; He, Hongju 4 ; Zhu, Qiang 1 ; Sun, Chuanlong 1 ; Wu, Ning 1 ; Zhang, Xiaofei 1 ; Huang, Tingting 3 ; Li, Chang-Bao 5 ; Li, Chuanyou 1 ; Deng, Lei 1 ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Innovat Acad Seed Design, Natl Ctr Plant Gene Res Beijing,State Key Lab Plan, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China

3.Qingdao Acad Agr Sci, Inst Vegetable, Qingdao 266100, Shandong, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr, Beijing 100097, Peoples R China

5.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2023 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Fruit color is an important horticultural trait, which greatly affects consumer preferences. In tomato, fruit color is determined by the accumulation of different pigments, such as carotenoids in the pericarp and flavonoids in the peel, along with the degradation of chlorophyll during fruit ripening. Since fruit color is a multigenic trait, it takes years to introgress all color-related genes in a single genetic background via traditional crossbreeding, and the avoidance of linkage drag during this process is difficult. Here, we proposed a rapid breeding strategy to generate tomato lines with different colored fruits from red-fruited materials by CRISPR/Cas9-mediated multiplex gene editing of three fruit color-related genes (PSY1, MYB12, and SGR1). Using this strategy, the red-fruited cultivar 'Ailsa Craig' has been engineered to a series of tomato genotypes with different fruit colors, including yellow, brown, pink, light-yellow, pink-brown, yellow-green, and light green. Compared with traditional crossbreeding, this strategy requires less time and can obtain transgene-free plants with different colored fruits in less than 1 year. Most importantly, it does not alter other important agronomic traits, like yield and fruit quality. Our strategy has great practical potential for tomato breeding and serves as a reference for improving multigene-controlled traits of horticultural crops.

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