您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Novel Self-Cleaving Viroid-Like RNA Identified in RNA Preparations from a Citrus Tree Is Not Directly Associated with the Plant

文献类型： 外文期刊

作者： Navarro, Beatriz 1 ; Li, Shuai 1 ; Gisel, Andreas 3 ; Chiumenti, Michela 1 ; Minutolo, Maria 5 ; Alioto, Daniela 5 ; Di Serio, Francesco 1 ;

作者机构： 1.Natl Res Council CNR, Inst Sustainable Plant Protect IPSP, Via Amendola 122-D, I-70126 Bari, Italy

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

3.CNR, Ist Tecnol Biomed, I-70126 Bari, Italy

4.Int Inst Trop Agr, Ibadan 200001, Nigeria

5.Univ Napoli Federico II, Dipartimento Agr, I-80055 Portici, Italy

关键词： circular RNAs; infectious non-coding RNAs; satellite RNAs; viroids; hammerhead ribozyme; self-cleavage; high-throughput sequencing

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Viroid and viroid-like satellite RNAs are infectious, circular, non-protein coding RNAs reported in plants only so far. Some viroids (family Avsunviroidae) and viroid-like satellite RNAs share self-cleaving activity mediated by hammerhead ribozymes (HHRzs) endowed in both RNA polarity strands. Using a homology-independent method based on the search for conserved structural motifs of HHRzs in reads and contigs from high-throughput sequenced RNAseq libraries, we identified a novel small (550 nt) viroid-like RNA in a library from a Citrus reticulata tree. Such a viroid-like RNA contains a HHRz in both polarity strands. Northern blot hybridization assays showed that circular forms of both polarity strands of this RNA (tentatively named citrus transiently-associated hammerhead viroid-like RNA1 (CtaHVd-LR1)) exist, supporting its replication through a symmetric pathway of the rolling circle mechanism. CtaHVd-LR1 adopts a rod-like conformation and has the typical features of quasispecies. Its HHRzs were shown to be active during transcription and in the absence of any protein. CtaHVd-LR1 was not graft-transmissible, and after its first identification, it was not found again in the original citrus source when repeatedly searched in the following years, suggesting that it was actually not directly associated with the plant. Therefore, the possibility that this novel self-cleaving viroid-like RNA is actually associated with another organism (e.g., a fungus), in turn, transiently associated with citrus plants, is proposed.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台