文献类型： 外文期刊
作者： Guo, Yanhua 1 ; Bai, Jiachen 1 ; Zhang, Zhenliang 1 ; Liu, Yucheng 1 ; Lu, Shouliang 1 ; Liu, Changbin 1 ; Ni, Jianhong 1 ; Zhou, Ping 1 ; Fu, Xiangwei 1 ; Sun, Wendell Q. 2 ; Wan, Pengcheng 1 ; Shi, Guoqing 1 ;
作者机构： 1.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Hlth Bree, Shihezi, Xinjiang, Peoples R China
2.Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Inst Biothermal Sci & Technol, Shanghai, Peoples R China
3.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Beijing Key Lab Anim Genet Improvement, Beijing, Peoples R China
关键词： ovine; embryo; cryopreserved methods; embryonic stages
期刊名称：CRYOLETTERS （ 影响因子：1.0； 五年影响因子：0.9 ）
ISSN： 0143-2044
年卷期： 2022 年 43 卷 5 期
页码：
收录情况： SCI
摘要： BACKGROUND: Developmental stage and cryopreservation method have significant impact on the pregnancy rate after transfer of embryos produced in vivo. OBJECTIVE: To determine the pregnancy outcomes from ovine embryos cryopreserved at different developmental stages. MATERIALS AND METHODS: Embryos at different developmental stages were obtained from donor ewes through simultaneous estrus treatment and laparoscopic artificial insemination. Embryos, either cryopreserved via vitrification or slow freezing method, were implanted into recipient ewes. The pregnancy rate was determined 35 days after transfer. RESULTS: The pregnancy rate of developing embryos increases after transfer from the morula stage, early blastocyst to expanded blastocyst stages (64.9%, 73.9% and 81.3%, respectively). However, cryopreservation significantly decreases the pregnancy rate of embryos at all three developmental stages, and there is no significant difference among developmental stages (43.9%, 43.7%, 52.9%, respectively). There is also no significant difference in the pregnancy rate between slowly-frozen embryos and vitrified embryos. CONCLUSION: The pregnancy outcomes of embryo transfer is better at the expanded blastocyst stage than at earlier stages. However, no difference is observed in the pregnancy rate of embryos at different developmental stage after cryopreservation, either by slow freezing and vitrification. Cryopreservation methods for ovine embryos, both slow freezing and vitrification, need further improvement.
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