A portable microfluidic chip-based fluorescent and colorimetric aptasensor combining recombinase polymerase amplification for bovine pregnancy-associated glycoproteins detection
文献类型： 外文期刊
作者： Feng, Jiaqi 1 ; Lu, Chunxia 4 ; Lin, Xianfeng 1 ; Kang, Lixin 1 ; Duan, Nuo 1 ; Wang, Zhouping 1 ; Liu, Changbin 5 ; Wu, Shijia 1 ;
作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Peoples R China
2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
3.Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China
4.Yangtze Normal Univ, Life Sci & Technol Inst, Chongqing 408100, Peoples R China
5.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi 832000, Peoples R China
关键词： Bovine milk monitoring; Microfluidic chip; Aptamer sensor; Recombinase polymerase amplification; Dual-mode detection
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.5； 五年影响因子：10.1 ）
ISSN： 0956-5663
年卷期： 2025 年 270 卷
页码：
收录情况： SCI
摘要： A portable dual-mode PDMS-based microfluidic chip aptasensor was developed to detect bovine pregnancyassociated glycoproteins (bPAG) in bovine milk. Reagents within the chip chambers underwent reactions driven by gravity, where pre-encoded rich C sequences on the complementary strand of the aptamer facilitated the generation of abundant G-quadruplexes via subsequent RPA reaction, which activated the chromogenic substrates and fluorogenic precursors in the chip, producing distinct colorimetric and fluorescent signals. These signals were captured by our developed smartphone application and converted into RGB values, further enabling the quantification of bPAG with detection limits of 0.079 ng/mL and 0.024 ng/mL for colorimetric and fluorescent modes, respectively, over a linear range of 0.1-100 ng/mL. Bovine milk and other animal source milk were evaluated in the proposed assay, accurate identification results were obtained, indicating significant potential in bovine milk monitoring. The work further provided a valuable reference for point-of-care testing of non-nucleic acid targets in food samples.
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