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Detection of acrylamide in food based on MIL-glucose oxidase cascade colorimetric aptasensor

文献类型： 外文期刊

作者： Guo, Kaixi 1 ; Lin, Xianfeng 1 ; Duan, Nuo 1 ; Lu, Chunxia 2 ; Wang, Zhouping 1 ; Wu, Shijia 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Resources, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, Shihezi 832000, Peoples R China

关键词： Metal-organic frameworks; Glucose oxidase; Cascade; Aptamer; Acrylamide

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0003-2670

年卷期： 2024 年 1288 卷

页码：

收录情况： SCI

摘要： Background: Maillard reaction involves the polymerization, condensation, and other reactions between compounds containing free amino groups and reducing sugars or carbonyl compounds during heat processing. This process endows unique flavors and colors to food, while it can also produce numerous hazards. Acrylamide (AAm) is one of Maillard's hazards with neurotoxicity and carcinogenicity, these effects can trigger mutations and alternations in gene expression in human cells and accelerate organ aging. An accurate and reliable acrylamide detection method with high sensitivity and specificity for future regulatory activities is urgently needed. Results: Herein, we constructed a colorimetric aptasensor with the hybridization of MIL-glucose oxidase (MGzyme)-cDNA and magnetic nanoparticle-aptamer (MNP-Apt) to specifically detect AAm. The incorporation of MB-Apt and AAm released MGzyme-cDNA in the supernatant, took the supernatant out, with the addition of glucose and TMB, MGzyme would oxidize glucose, the resulting center dot OH facilitated the oxidation of colorless TMB to blue ox-TMB. The absorbance value at 652 nm, which indicates the characteristic absorption peak of ox-TMB, exhibited a proportion to the concentration of AAm. MGzyme avoided the addition of harmful intermediate H2O2 and created an acid microenvironment for the catalytic reaction. MNP-Apt possessed the advantages of high specificity and simplified separation. Under optimal conditions, this method displayed a linear range of 0.01-100 mu M with the limit of detection of 1.53 nM. With the spiked analysis data cross-verified by ELISA kit, this aptasensor was proven to specifically detect AAm at low concentrations.

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