Structural optimization, characterization, and evaluation of binding mechanism of aptamers against bovine pregnancy-associated glycoproteins and their application in establishment of a colorimetric aptasensor using Fe-based metal-organic framework as peroxidase mimic tags
文献类型： 外文期刊
作者： Lu, Chunxia 1 ; Qin, Jiaxiang 3 ; Wu, Shijia 4 ; Zhang, Zhenliang 2 ; Tang, Zonggui 2 ; Liu, Changbin 2 ;
作者机构： 1.Yangtze Normal Univ, Life Sci & Technol Inst, Chongqing 408100, Peoples R China
2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, Shihezi 832000, Peoples R China
3.Sun Yat Sen Univ, Sch Mat & Engn, Key Lab Low Carbon Chem & Energy Conservat Guangdo, Guangzhou 510275, Peoples R China
4.Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
关键词： Pregnancy-associated glycoproteins; Binding mechanism; Molecular dynamics; Colorimetric aptasensor; Metal-organic frameworks
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）
ISSN： 0026-3672
年卷期： 2024 年 191 卷 11 期
页码：
收录情况： SCI
摘要： A truncated aptamer (designated A24-3) was identified that specifically binds to bovine pregnancy-associated glycoproteins (bPAG9) with a low dissociation constant (2.04 nM) through two truncation approaches. Circular dichroism spectroscopy indicated that A24-3 formed parallel G-quadruplexes, which was subsequently confirmed using nuclear magnetic resonance (NMR) spectroscopy. Furthermore, a molecular dynamics simulation was employed to investigate the recognition mechanism of A24-3 and bPAG9. Interaction analysis showed that A24-3 folded into a parallel G-quadruplex structure with three G-tetrads, primarily through numerous hydrogen bonds and hydrophobic and pi-pi interactions. Finally, a novel colorimetric aptasensor was developed for detecting bPAG9 using A24-3 and an Fe-based metal-organic framework as target recognition elements and enzyme mimics, respectively. The method demonstrated a broad detection range from 0.5 to 50 ng/mL, with a low detection limit of 0.03 ng mL-1, and exhibited a good recovery (91.0-102%) for bPAG9-spiked serum samples. Additionally, the aptasensor was successfully applied to detecting the pregnancy-specific biomarker bPAGs in serum samples.
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