Ratiometric SERS aptasensing for simultaneous quantitative detection of histamine and tyramine in fishes
文献类型： 外文期刊
作者： Duan, Nuo 1 ; Chang, Yuting 1 ; Lv, Wenhui 1 ; Li, Changxin 1 ; Lu, Chunxia 2 ; Wang, Zhouping 1 ; Wu, Shijia 1 ;
作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Resources, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China
2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, Shihezi 83200, Peoples R China
关键词： Histamine; Tyramine; Aptasensor; SERS; Ratiometric
期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）
ISSN： 0039-9140
年卷期： 2023 年 265 卷
页码：
收录情况： SCI
摘要： Herein, a SiO2@Ag NPs core/shell nanoparticles were synthesized to fabricate a surface-enhanced Raman spectroscopy (SERS) sensor for the simultaneous determination of histamine (HIS) and tyramine (TYR) based on specific aptamer recognition and ratiometric strategy. SiO2@Ag NPs with 4-thiosaminophenol (4-ATP) and Nile blue A (NBA) molecules were used as an internal standard (IS) and labeled with aptamers corresponding to HIS and TYR, respectively. Raman reporter molecules ROX and Cy5 labeled complementary DNA (cDNA) were then hybridized with aptamers to form rigid double-stranded DNA. After the HIS and TYR were captured by their aptamers, resulting in the dissociation of cDNA and separated from the SERS substrate. Therefore, the SERS signal intensity at 1503 cm-1 of ROX and 1358 cm-1 of Cy5 tagged on the terminal of cDNA decreased with the concentration of HIS and TYR increasing, while the SERS signal intensity at 1079 cm-1 of 4-APT and 592 cm-1 of NBA on the substrate remain stable. Thus, the concentrations of HIS and TYR can be determined by the I1503/ I1079 and I1358/I592 values, respectively. This sensing strategy achieves a lower detection limit of 0.2 ng/mL for HIS and 0.05 ng/mL for TYR, respectively, demonstrating promising applications in sensitive detection of BAs in animal-derived foodstuff.
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