Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)
文献类型： 外文期刊
作者： Li, Yanan 1 ; Chen, Zongfu 1 ; Zhang, Peng 2 ; Gao, Feng 1 ; Wang, Junfeng 3 ; Lin, Li 1 ; Zhang, Haibin 4 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
关键词： Holothuroidea; deep sea; enzyme expression; purification
期刊名称：ANTIOXIDANTS （ 影响因子：7.0； 五年影响因子：7.3 ）
ISSN：
年卷期： 2023 年 12 卷 6 期
页码：
收录情况： SCI
摘要： At present, deep-sea enzymes are a research hotspot. In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 & DEG;C, and it maintains high activity in the range of 0-60 degrees C. It also has high thermal stability when incubated at 37 & DEG;C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4-11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.
- 相关文献
作者其他论文 更多>>
-
Impact of Climate Change on the Habitat Distribution of Decapterus macarellus in the South China Sea
作者：Shen, Qikun;Zhang, Peng;Yu, Wenming;Cai, Yancong;Li, Jie;Chen, Zuozhi;Fan, Jiangtao;Shen, Qikun;Zhang, Peng;Yu, Wenming;Cai, Yancong;Li, Jie;Chen, Zuozhi;Fan, Jiangtao;Zhang, Peng;Chen, Zuozhi;Xiong, Pengli
关键词：SDMs;
Decapterus macarellus ; the South China Sea; climate change; environmental factors -
Enhanced virulence of Acinetobacter johnsonii at low temperatures induces acute immune response and systemic infection in American bullfrogs (Aquarana catesbeiana)
作者：Lin, Han;Sun, Jingyang;Li, Hua;Chen, Kesong;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Cold stress; Virulence genes; Amphibian disease; Immune response; Systemic infection
-
Unravelling the pathogenesis of Vibrio parahaemolyticus infection: impacts on antioxidant and innate immunity gene responses in Scylla paramamosain
作者：Liu, Guangxin;Fan, Sigang;Ma, Hongling;Deng, Yiqin;Jiang, Jianjun;Cheng, Changhong;Feng, Juan;Guo, Zhixun;Lin, Li;Qin, Zhendong;Li, Ce
关键词：Scylla paramamosain; V. parahaemolyticus LG2206; Oxidative stress; Immune response; Tissue pathogenesis
-
A Field Trial to Study the Effects of Stenella attenuata Deterrent on the Catch of a Light-Falling Net Fishery
作者：Wang, Teng;Li, Jie;Yan, Lei;Zhang, Peng;Li, Min;Xie, Bin;Song, Zhongchang;Fu, Weijie;Zhang, Yu
关键词：Stenella attenuata; acoustic deterrent device; fish catch; generalized additive model (GAM)
-
Exploring the Habitat Distribution of Decapterus macarellus in the South China Sea Under Varying Spatial Resolutions: A Combined Approach Using Multiple Machine Learning and the MaxEnt Model
作者：Shen, Qikun;Zhang, Peng;Feng, Xue;Chen, Zuozhi;Fan, Jiangtao;Shen, Qikun;Zhang, Peng;Chen, Zuozhi;Fan, Jiangtao;Zhang, Peng;Chen, Zuozhi
关键词：
Decapterus macarellus ; spatial resolution; machine learning; SHAP; MaxEnt -
Characterization of a trypanosome from large yellow croaker (Larimichthys crocea), cage-cultured in seawater, in China
作者：Wang, Ju-Feng;Li, Xin-Tao;Zhang, Peng;Lai, De-Hua;Lun, Zhao-Rong;Xu, Li-Wen;Zhang, Jin-Yong;Hide, Geoff;Lun, Zhao-Rong;Hide, Geoff;Lun, Zhao-Rong
关键词：Trypanosoma carassii; Fish trypanosomosis; Large yellow croaker; Marine fish
-
Archidendron clypearia extract: Modulating intestinal inflammation and enhancing disease resistance in aquacultured American bullfrogs (Aquarana catesbeiana)
作者：Chen, Kesong;Lin, Han;Sun, Jingyang;Li, Hua;Ma, Jie;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing
关键词：American bullfrogs; Archidendron clypearia; Disease resistance; Intestinal inflammation
