Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)
文献类型： 外文期刊
作者： Li, Yanan 1 ; Chen, Zongfu 1 ; Zhang, Peng 2 ; Gao, Feng 1 ; Wang, Junfeng 3 ; Lin, Li 1 ; Zhang, Haibin 4 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
关键词： Holothuroidea; deep sea; enzyme expression; purification
期刊名称：ANTIOXIDANTS （ 影响因子：7.0； 五年影响因子：7.3 ）
ISSN：
年卷期： 2023 年 12 卷 6 期
页码：
收录情况： SCI
摘要： At present, deep-sea enzymes are a research hotspot. In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 & DEG;C, and it maintains high activity in the range of 0-60 degrees C. It also has high thermal stability when incubated at 37 & DEG;C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4-11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.
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