Nanobody-based fluorescent immunoassay using carbon dots anchored cobalt oxyhydroxide composite for the sensitive detection of fenitrothion
文献类型： 外文期刊
作者： Luo, Lin 1 ; Lin, Shi-Qi 1 ; Wu, Zhuo-Yu 1 ; Wang, Hong 1 ; Chen, Zi-Jian 1 ; Deng, Hao 2 ; Shen, Yu-Dong 1 ; Zhang, Wen-Feng 3 ; Lei, Hong-Tao 1 ; Xu, Zhen-Lin 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
2.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold Chain Hainan P, Haikou 570100, Peoples R China
3.Guangdong Acad Sci, Inst Anal, Guangdong Prov Engn Res Ctr Rapid Testing Instrume, China Natl Analyt Ctr,Guangdong Prov Key Lab Chem, Guangzhou 510070, Peoples R China
关键词： Organophosphorus pesticides; Immunoassay; Forster resonance energy transfer; Nanobody; Carbon dots
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：14.224； 五年影响因子：12.984 ）
ISSN： 0304-3894
年卷期： 2022 年 439 卷
页码：
收录情况： SCI
摘要： Fenitrothion (FN) residue in food is a serious threat to public health. Consequently, a sensitive, cost-effective, and convenient immunoassay for FN urgently needs to be fabricated to safeguard human health. Herein, a nanobody-alkaline phosphatase fusion protein (Nb-ALP)-based fluorescent ELISA using red emissive carbon dots (r-CDs) anchored cobalt oxyhydroxide nanosheet (CoOOH NS) composite was developed for detecting FN. Briefly, a Nb-ALP was obtained by autoinduction expression and employed as a recognition, signal transduction, and ampli-fication element. As the fluorescence signal source, r-CDs were assembled with CoOOH NS to yield the r-CDs@CoOOH NS composite, leading to the fluorescence quenching of r-CDs via Fo spacing diaeresis rster resonance energy transfer (FRET). After competitive immunoreaction, the Nb-ALP bounded to the immobilized antigen can mediate the production of ascorbic acid, which can reduce the CoOOH NS to Co2+, breaking the FRET between r-CDs and CoOOH NS, accompanied by the fluorescence recovery of r-CDs. This fluorescent ELISA is highly sensitive to FN with a detection limit of 0.14 ng mL-1, which is 25-fold lower than that of conventional colorimetric ELISAs. The recovery test of food samples and the validation by GC-MS/MS further demonstrated the proposed assay was an ideal tool for detecting FN.
- 相关文献
作者其他论文 更多>>
-
Development and Assessment of a Color-Variable Chlorine Dioxide Slow-Releasing Card for Litchi Preservation
作者：Guo, Li;Luo, Lin;Deng, Hao;Guo, Li;Wu, Guang;Deng, Hao;Wu, Guang;Deng, Hao;Wu, Guang;Deng, Hao;Yin, Qingchun
关键词：ClO2 slow-releasing card; color; litchi; development; assessment
-
Antibacterial and antioxidant plant-derived aldehydes: A new role as cross-linking agents in biopolymer-based food packaging films
作者：Deng, Hao;Zhang, Wanli;Huang, Zhaoxian;Ramezan, Yousef;Riahi, Zohreh;Khan, Ajahar;Riahi, Zohreh;Khan, Ajahar
关键词：cross-linking; food packaging; plant aldehydes; Schiff base
-
Preparation and evaluation of ozone micro-nano bubbles ice for Litchi precooling
作者：Deng, Hao;Guo, Li;Wu, Guang;Deng, Hao;Wu, Guang;Deng, Hao;Wu, Guang;Chen, Zhe;Abbas, Farhat;Yin, Qingchun
关键词：Ozone; Ice; Micro-Nano; Litchi; Precooling
-
Application of tannic acid and Fe3+crosslinking-enhanced chitosan films for wax apple (Syzygium samarangense) preservation
作者：Guo, Junyan;Zhang, Zhengke;Zhang, Wanli;Goksen, Gulden;Khan, Mohammad Rizwan;Ahmad, Naushad;Deng, Hao
关键词：Wax apple; Edible coating; Antioxidant properties
-
Konjac glucomannan/ carboxylated cellulose nanofiber-based edible coating with tannic acid maintains quality and prolongs shelf-life of mango fruit
作者：Wang, Jiaxin;Qin, Mian;Wang, Wei;Xia, Yining;Lin, Qiong;Xia, Yining;Lin, Qiong;Wu, Guang;Deng, Hao
关键词：Coating; Konjac glucomannan; Carboxylated cellulose nanofiber; Tannic acid; Mango
-
Chitosan-platinum nanozyme-mediated ratiometric fluorescent immunosensor with dual catalytic cascades for sensitive detection of fenitrothion
作者：Liu, Jie;Deng, Hao;Jia, Bao-Zhu;Liu, Jie;Luo, Lin;Wang, Hong;Xu, Zhen-Lin;Yin, Qing-Chun;Wu, Min-Fu
关键词：Nanozyme; Catalytic cascades; Ratiometric fluorescence; Immunosensor
-
Microbial contribution to 14 biogenic amines accumulation in refrigerated raw and deep-fried hairtails (Trichiurus lepturus)
作者：Deng, Hao;Wu, Guang;Guo, Li;Luo, Senlin;Yin, Qingchun;Deng, Hao;Zhou, Lingyu;Chen, Xiaomei;Yin, Qingchun;Deng, Hao;Luo, Senlin
关键词：UPLC -MS/MS; Biogenic amine; Hairtail; Bacterium; Storage
