Nanobody-based fluorescent immunoassay using carbon dots anchored cobalt oxyhydroxide composite for the sensitive detection of fenitrothion
文献类型： 外文期刊
作者： Luo, Lin 1 ; Lin, Shi-Qi 1 ; Wu, Zhuo-Yu 1 ; Wang, Hong 1 ; Chen, Zi-Jian 1 ; Deng, Hao 2 ; Shen, Yu-Dong 1 ; Zhang, Wen-Feng 3 ; Lei, Hong-Tao 1 ; Xu, Zhen-Lin 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
2.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold Chain Hainan P, Haikou 570100, Peoples R China
3.Guangdong Acad Sci, Inst Anal, Guangdong Prov Engn Res Ctr Rapid Testing Instrume, China Natl Analyt Ctr,Guangdong Prov Key Lab Chem, Guangzhou 510070, Peoples R China
关键词： Organophosphorus pesticides; Immunoassay; Forster resonance energy transfer; Nanobody; Carbon dots
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：14.224； 五年影响因子：12.984 ）
ISSN： 0304-3894
年卷期： 2022 年 439 卷
页码：
收录情况： SCI
摘要： Fenitrothion (FN) residue in food is a serious threat to public health. Consequently, a sensitive, cost-effective, and convenient immunoassay for FN urgently needs to be fabricated to safeguard human health. Herein, a nanobody-alkaline phosphatase fusion protein (Nb-ALP)-based fluorescent ELISA using red emissive carbon dots (r-CDs) anchored cobalt oxyhydroxide nanosheet (CoOOH NS) composite was developed for detecting FN. Briefly, a Nb-ALP was obtained by autoinduction expression and employed as a recognition, signal transduction, and ampli-fication element. As the fluorescence signal source, r-CDs were assembled with CoOOH NS to yield the r-CDs@CoOOH NS composite, leading to the fluorescence quenching of r-CDs via Fo spacing diaeresis rster resonance energy transfer (FRET). After competitive immunoreaction, the Nb-ALP bounded to the immobilized antigen can mediate the production of ascorbic acid, which can reduce the CoOOH NS to Co2+, breaking the FRET between r-CDs and CoOOH NS, accompanied by the fluorescence recovery of r-CDs. This fluorescent ELISA is highly sensitive to FN with a detection limit of 0.14 ng mL-1, which is 25-fold lower than that of conventional colorimetric ELISAs. The recovery test of food samples and the validation by GC-MS/MS further demonstrated the proposed assay was an ideal tool for detecting FN.
- 相关文献
作者其他论文 更多>>
-
Microbial contribution to 14 biogenic amines accumulation in refrigerated raw and deep-fried hairtails (Trichiurus lepturus)
作者：Deng, Hao;Wu, Guang;Guo, Li;Luo, Senlin;Yin, Qingchun;Deng, Hao;Zhou, Lingyu;Chen, Xiaomei;Yin, Qingchun;Deng, Hao;Luo, Senlin
关键词：UPLC -MS/MS; Biogenic amine; Hairtail; Bacterium; Storage
-
Development of broad-spectrum immunoassay with monoclonal antibody to detect five eugenols and study of their molecular recognition mechanism
作者：Luo, Lin;Kang, Rui-Yao;He, Zhen-Xi;Chen, Zi-Jian;Xu, Zhen-Lin;Deng, Hao;Luo, Lin;Xu, Zhen-Lin;Chen, Zi-Jian;Jia, Bao-Zhu
关键词：Immunoassay; Hapten; Monoclonal antibody; Eugenol; Fish anesthetic
-
Simulated logistical transport effects on textural change of wax apple using different packaging types during storage time
作者：Wei, Wanqi;Li, Baoqing;Zhao, Tong;Cheng, Chiyun;Yan, Ruixiang;Deng, Hao;Zhang, Na;Chen, Cunkun
关键词：Wax apple; Textural change; Packaging type; Simulated transport; Storage time
-
A review of starch/polyvinyl alcohol (PVA) blend film: A potential replacement for traditional plastic-based food packaging film
作者：Deng, Hao;Su, Jiaqi;Zhang, Wanli;Khan, Ajahar;Rhim, Jong-Whan;Khan, Ajahar;Rhim, Jong-Whan;Sani, Mahmood Alizadeh;Goksen, Gulden;Kashyap, Piyush;Ezati, Parya
关键词：Biopolymer; Biodegradable; Food preservation; Coating
-
Integrated metabolomic and transcriptomic analyses reveal quality differences between artificially and naturally ripened Guifei mangoes
作者：Deng, Hao;Wu, Guang;Chen, Zhe;Guo, Li;Yin, Qingchun;Zhang, Wanli;Deng, Hao;Wu, Guang;Chen, Zhe;Deng, Hao;Yin, Qingchun;Zhang, Wanli
关键词：Guifei mango; Metabolome; Transcriptome; Ripening; Quality
-
Supplementation of dietary areca nut extract modulates the growth performance, cecal microbiota composition, and immune function in Wenchang chickens
作者：Wang, Shiping;Dai, Jiahui;Dai, Wenting;Kang, Xiaoning;Wang, Hong;Jiang, Qicheng;Zheng, Xinli;He, Zhongchun;Gu, Lihong;Xu, Tieshan;Fu, An;Xing, Zengyang;Chen, Yiyong;Lu, Lizhi
关键词：Wenchang chickens; Areca catechu; growth performance; metabonomics; cecal microbiota; MHC-BF1; Daxx upon analysis
-
High-throughput detection and dietary exposure risk assessment of 44 mycotoxins in Mango, Litchi, Longan, and their products in South China
作者：Deng, Hao;Yin, Qingchun;Deng, Hao;Gao, Yunkai;Zhou, Lingyu;Chen, Xiaomei;Chen, Chunquan;Li, Bei;Yin, Qingchun;Deng, Hao;Xu, Zhenlin;Luo, Lin
关键词：UPLC-MS/MS; Mycotoxins; Mango; Lichi; Longan; Dietary exposure risk
