文献类型： 外文期刊

作者： Sun Xiaowen ¹ ; Liang Liqun ¹ ; Welt, Marc ¹ ; Jia Zhiying ¹ ; Chang Yumei ¹ ; Lu Chuiyun ¹ ; Yan Xuechun ¹ ; Lv Xin ¹ ; Lei ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Minist Agr, Harbin 150070, Peoples R China

期刊名称：PROCEEDINGS OF THE 13TH INTERNATIONAL CONGRESS ON GENES, GENE FAMILIES AND ISOZYMES

ISSN：

年卷期： 2005 年

页码：

收录情况： SCI

摘要： The microsatellite DNA from seven main fresh water aquatic species including common carp, silver crucian carp, grass carp, silver carp, bighead carp, snout bream, Chinese river crab were cloned by two methods. One method is screening short inserted DNA library using a few synthetic oligonucleotide simple repeated sequence labeled at the 5'end with [gamma-p(32)] ATP. The other method is based on using biotinylated probes attached to magnetic beads to isolate pieces of DNA containing the microsatellites. We got 1180 microsatellite DNA for common carp, 137 for silver crucian carp, 114 for grass carp, 138 for silver carp,75 for bighead carp, 108 for Chinese river crab, 120 for snout bream. PCR primers were designed using soft ware PRIMER5. A total of 210 primer sets were tested for polymorphism on each species panels of 6 animals. 60% markers were found to be polymorphic. These polymorphic markers as well as non-polymorphic markers will be very valuable as genetic tools for using in strain identification, parentage analysis, quantity trait loci analysis, population structure analysis and marker-based selection studies.