文献类型： 外文期刊

作者： Wang, CY ¹ ; Duan, YR ² ; Markovic, B ² ; Barbara, J ² ; Howlett, CR ² ; Zhang, XD ² ; Zreiqat, H ² ;

作者机构： 1.Sichuan Univ, Engn Res Ctr Biomat, Chengdu 610064, Peoples R China

2.Sichuan Univ, Engn Res Ctr Biomat, Chengdu 610064, Peoples R China; Chinese Acad Fishery Sci, Yangtze Fisheries Inst, Jinzhou 434000, Hubei Province, Peoples R China; Univ New S Wales, Sch Safety Sci, Chem Safety & Toxicol Labs, Sydney, NSW 2052, Australia; Univ New S Wales, Dept Pathol, Bone Biomat Unit, Sydney, NSW 2052, Australia

关键词： calcium phosphate ceramics;phase composition;cell proliferation;bone-related genes;phenotypic expression

期刊名称：BIOMATERIALS （ 影响因子：12.479； 五年影响因子：12.104 ）

ISSN： 0142-9612

年卷期： 2004 年 25 卷 13 期

页码：

收录情况： SCI

摘要： Calcium phosphate ceramics with different hydroxyapatite (HA) and tricalcium phosphate (TCP) ratios have different chemical properties. Does the difference in phase composition affect osteoblast behavior? In this study, osteoblasts were cultured on 4 kinds of calcium phosphate ceramics, i.e. pure (HA), HT1 (HA/TCP, 70/30), HT2 (HA/TCP, 35/65), and pure TCP. Cell proliferation of SaOS-2 cells together with bone-related genes' mRNA expression and protein production in osteoblasts cultured on different calcium phosphate ceramics were detected at different time points. Data suggested that cell proliferation rate on TCP ceramics was lower than that on the other substrates tested. Generally, mRNA expressions for ostenectin and osteocalcin were similar among the four kinds of ceramics in most circumstances, whereas at six days, alkaline phosphatase mRNA expression was higher on HA and HT1 surfaces than on the other two materials. Collagen I mRNA expression was also affected by the phase composition of substrates. Osteocalcin and bone sialoprotein production in SaOS-2 cells was very similar no matter which ceramic surface the cells were grown upon. This study revealed that calcium phosphate ceramics substrate could support osteoblast growth and bone-related gene expression and its gene expression pattern explained the basis of the biocompatibility and bioactivity for calcium phosphate ceramics. (C) 2003 Published by Elsevier Ltd.