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Analysis of the genetic diversity of different populations of Pinctada maxima from China

文献类型： 外文期刊

作者： Fang, Wei 1 ; Chen, Mingqiang 1 ; Zhao, Wang 4 ; Wang, Yu 1 ; Ma, Zhenhua 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Peoples R China

2.South China Sea Fisheries Res Inst, Trop Fisheries Res & Dev Ctr, Guangzhou, Peoples R China

3.South China Sea Fisheries Res Inst, Sanya Trop Fisheries Res Inst, Guangzhou, Peoples R China

4.South China Sea Fisheries Res Inst, Guangzhou, Peoples R China

关键词： Pinctada maxima; SSR primer; genetic diversity; genetic differentiation

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.4； 五年影响因子：0.5 ）

ISSN： 0792-156X

年卷期： 2025 年 77 卷 1 期

页码：

收录情况： SCI

摘要： This study aims to analyze the genetic diversity and differences among wild populations of Pinctada maxima, providing a foundational reference for the enhancement and optimization of its germplasm. In this study, we developed SSR primers based on simplified genome sequencing and screened 10 pairs using fluorescent PCR amplification to assess the genetic diversity of wild populations of P. maxima from Danzhou (DZ), Nansha (NS), and Xuwen (XW), China. Additionally, we investigated the genetic richness and the degree of differentiation among the populations. The results indicated that the number of alleles (Na) detected by the 10 SSR primer pairs ranged from 8 to 17 across the three P. maxima populations, with a total of 130 alleles. The polymorphic information content (PIC) varied from 0.769 to 0.865, with an average of 0.8531. All ten pairs of SSR primers exhibited high levels of polymorphism. The observed heterozygosity (Ho) among the three P. maxima populations ranged from 0.1333 to 1.000, with an average of 0.7884. The expected heterozygosity (He) ranged from 0.6333 to 0.8755, with an average of 0.7815. The mean values of Hardy-Weinberg equilibrium indices (F) ranged from -0.032 to 0.058, indicating that all populations were approximately in Hardy-Weinberg equilibrium, with the F values for DZ and XW populations being negative, suggesting a heterozygote deficit. The mean Fst value for the three P. maxima populations was 0.098, and molecular ANOVA revealed that 10% of the genetic differentiation occurred between populations, while 90% arose from individual variation. The average gene flow (Nm) between populations was 2.804, suggesting that genetic drift did not dominate the changes in population genetic structure. Among the three populations, the genetic distance between the DZ and XW populations was the smallest (0.3352), while the NS and DZ populations exhibited the greatest genetic distance (0.6462). UPGMA clustering analysis based on genetic distances revealed that the three P. maxima populations could be divided into two major groups, with the DZ and XW populations clustering independently into one group. Significant genetic differentiation and high genetic diversity were observed among the three P. maxima populations, although heterozygous diversity of the offspring can be enhanced by introducing these three populations, each affinities, as parent stock.

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