Detection of amino thiols from fish samples by stable isotope chemical labelling coupled with ultra-performance liquid chromatography-tandem mass spectrometry
文献类型： 外文期刊
作者： Wu, Siyuan 1 ; Feng, Yang 1 ; Chen, Shengjun 1 ; Zhao, Yongqiang 1 ; Deng, Jianchao 1 ; Wang, Di 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Natl R&D Ctr Aquat Prod Proc, Key Lab Aquat Prod,Minist Agr & Rural Affairs, Guangzhou 510330, Peoples R China
2.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572426, Peoples R China
3.Shanghai Ocean Univ, Coll Food Sci, Shanghai 201306, Peoples R China
关键词： Amino thiols; Liquid extraction; Iodoacetamide; Stable isotope labeling; UPLC-MS; Transportation stress
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 471 卷
页码：
收录情况： SCI
摘要： The amino thiols are key antioxidants in organisms, and their detection in food is of significant importance. This study developed a new stable isotope chemical labelling coupled with ultra-performance liquid chromatography- tandem mass spectrometry method to detect six amino thiols from fish samples. By the proposed method, amino thiols were labeled after liquid extraction using the stable isotope labeling reagents of iodoacetamide (IAM) and D4-IAM. Desirable recovery and linearity of thiols were achieved in fish samples. The limits of detection (LOD) ranged from 0.2 to 6.5 nmol/g, and the detection sensitivity was improved from 2.6 to 74 folds. The cluster analysis revealed that this method successfully discriminated eight fish species and their sources. Additionally, we found that glutathione and cysteine were significantly correlated with the transportation stress status of golden pompano ( Trachinotus ovatus). Overall, this study presents a sensitive and efficient method for the detection of amino thiols from fish samples.
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