文献类型： 外文期刊

作者： Feng, X. ¹ ; Memon, F. U. ¹ ; Ma, C. ¹ ; Zhu, J. ³ ; Huang, Z. ¹ ; Tettamanti, G. ⁵ ; Li, K. ⁴ ; Peng, G. ¹ ; Hu, W. ¹ ; Tian, L. ¹ ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China

2.Shaoguan Univ, Sch Biol & Agr, Shaoguan 512005, Peoples R China

3.Bioforte Biotechnol Shenzhen Co Ltd, Shenzhen 518118, Peoples R China

4.South China Normal Univ, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Guangzhou 510631, Peoples R China

5.Univ Insubria, Dept Biotechnol & Life Sci, I-21100 Varese, Italy

6.Univ Napoli Federico II, Interuniv Ctr Studies Bioinspired Agroenvironm Te, I-80055 Portici, Italy

7.South China Normal Univ, Guangmeiyuan R&D Ctr, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Meizhou 514779, Peoples R China

8.South China Agr Univ, Appl Microbiol Lab, Coll Food Sci, Guangzhou 510642, Guangdong, Peoples R China

9.Hechi Univ, Guangxi Collaborat Innovat Ctr Modern Sericulture, Guangxi Key Lab Sericulture Ecol & Appl Intellige, Hechi 546300, Peoples R China

关键词： cold/heat tolerance; full-length transcriptome; Hermetia illucens; proteome; RNA-seq

期刊名称：JOURNAL OF INSECTS AS FOOD AND FEED （ 影响因子：3.5； 五年影响因子：4.9 ）

ISSN：

年卷期： 2025 年 11 卷 3 期

页码：

收录情况： SCI

摘要： The larvae of black soldier fly (BSFL) can convert organic waste into insect proteins. Their bioconversion performance is considerably reduced under hot and cold conditions. To address this problem, we performed selective breeding of BSFL at 16 degrees C (T16) and 40 degrees C (T40), and evaluated differentially expressed genes and proteins in the heat- and cold-tolerant breeds. Both the T16 and T40 breeds exhibited higher body weight, survival rate, and substrate reduction efficiency compared to the control groups (C16 and C40). Transcriptome analysis showed significant gene expression changes in both the T16 and T40 groups at various time points compared to the control groups. Specifically, the T16 group had 84 upregulated and 64 downregulated genes, while the T40 group had 117 upregulated and 483 downregulated genes. Full-length transcriptome analyses revealed 27,414 new transcripts in the T16 group. Proteomic analysis revealed 57 upregulated and 114 downregulated proteins in the T16 group, and 128 upregulated and 68 downregulated in the T40 group. The upregulated genes, proteins, and novel transcripts in the bred strains mainly participated in Toll and Imd signalling, carbon metabolism, carbohydrate metabolism, fatty acid biosynthesis and metabolism, and amino acid metabolism. These findings provide insights into the molecular mechanisms that help BSFL adapt to cold and heat stress.