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Metagenomic Insight into Lignocellulose Degradation of the Thermophilic Microbial Consortium TMC7

文献类型： 外文期刊

作者： Wang, Yi 1 ; Wang, Chen 1 ; Chen, Yonglun 1 ; Chen, Beibei 1 ; Guo, Peng 1 ; Cui, Zongjun 3 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol Res, Wuhan 430064, Peoples R China

2.Three Gorges Univ, Coll Biol & Pharm, Yichang 443002, Peoples R China

3.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China

关键词： Llignocellulose degradation; microbial consortium; metagenomics; CAZymes; sugar cheater

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN： 1017-7825

年卷期： 2021 年 31 卷 8 期

页码：

收录情况： SCI

摘要： Biodegradation is the key process involved in natural lignocellulose biotransformation and utilization. Microbial consortia represent promising candidates for applications in lignocellulose conversion strategies for biofuel production; however, cooperation among the enzymes and the labor division of microbes in the microbial consortia remains unclear. In this study, metagenomic analysis was performed to reveal the community structure and extremozyme systems of a lignocellulolytic microbial consortium, TMC7. The taxonomic affiliation of TMC7 metagenome included members of the genera Ruminiclostridium (42.85%), Thermoanaerobacterium (18.41%), Geobacillus (10.44%), unclassified_f__Bacillaceae (7.48%), Aeribacillus (2.65%), Symbiobacterium (2.47%), Desulfotomaculum (2.33%), Caldibacillus (1.56%), Clostridium (1.26%), and others (10.55%). The carbohydrate-active enzyme annotation revealed that TMC7 encoded a broad array of enzymes responsible for cellulose and hemicellulose degradation. Ten glycoside hydrolases (GHs) endoglucanase, 4 GHs exoglucanase, and 6 GHs beta-glucosidase were identified for cellulose degradation; 6 GHs endo-beta-1,4-xylanase, 9 GHs beta-xylosidase, and 3 GHs beta-mannanase were identified for degradation of the hemicellulose main chain; 6 GHs arabinofuranosidase, 2 GHs alpha mannosidase, 11 GHs galactosidase, 3 GHs alpha-rhamnosidase, and 4 GHs alpha-fucosidase were identified as xylan debranching enzymes. Furthermore, by introducing a factor named as the contribution coefficient, we found that Ruminiclostridium and Thermoanaerobacterium may be the dominant contributors, whereas Symbiobacterium and Desulfotomaculum may serve as "sugar cheaters" in lignocellulose degradation by TMC7. Our findings provide mechanistic profiles of an array of enzymes that degrade complex lignocellulosic biomass in the microbial consortium TMC7 and provide a promising approach for studying the potential contribution of microbes in microbial consortia.

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