您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

文献类型： 外文期刊

作者： Wang, Qian-Qian 1 ; Zhu, Meng-Jia 2 ; Yu, Xia 1 ; Bi, Yuan-Yang 1 ; Zhou, Zhuang 1 ; Chen, Ming-Kun 1 ; Chen, Jiating 1 ; Zhang, Diyang 1 ; Ai, Ye 1 ; Liu, Zhong-Jian 1 ; Lan, Siren 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Natl Forestry & Grassland Adm Orchid Cons, Fuzhou, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou, Peoples R China

3.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou, Peoples R China

4.Shandong Acad Agr Sci, Inst Vegetable & Flowers, Jinan, Peoples R China

关键词： terpenes; terpene synthase; orchids; floral scent; expression analysis; Cymbidium

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Terpene synthases (TPSs) are essential for forming terpenes, which play numerous functional roles in attracting pollinators, defending plants, and moderating the interaction between plants. TPSs have been reported in some orchids, but genome-wide identification of terpenes in Cymbidium faberi is still lacking. In this study, 32 putative TPS genes were classified in C. faberi and divided into three subfamilies (TPS-a, TPS-b, and TPS-e/f). Motif and gene structure analysis revealed that most CfTPS genes had the conserved aspartate-rich DDxxD motif. TPS genes in the TPS-a and TPS-b subfamilies had variations in the RRX8W motif. Most cis-elements of CfTPS genes were found in the phytohormone responsiveness category, and MYC contained most of the numbers associated with MeJA responsiveness. The Ka/Ks ratios of 12/13 CfTPS gene pairs were less than one, indicated that most CfTPS genes have undergone negative selection. The tissue-specific expression patterns showed that 28 genes were expressed in at least one tissue in C. faberi, and TPS genes were most highly expressed in flowers, followed by leaves and pseudobulbs. In addition, four CfTPS genes were selected for the real-time reverse transcription quantitative PCR (RT-qPCR) experiment. The results revealed that CfTPS12, CfTPS18, CfTPS23, and CfTPS28 were mainly expressed in the full flowering stage. CfTPS18 could convert GPP to beta-myrcene, geraniol, and alpha-pinene in vitro. These findings of CfTPS genes of C. faberi may provide valuable information for further studies on TPSs in orchids.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台