Separation of Heat-Stable Antifungal Factor From Lysobacter enzymogenes Fermentation Broth via Photodegradation and Macroporous Resin Adsorption
文献类型： 外文期刊
作者： Tang, Bao 1 ; Wu, Lingtian 3 ; Wang, Jinzi 1 ; Sun, Weibo 1 ; Zhao, Yancun 1 ; Liu, Fengquan 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing, Peoples R China
2.Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang, Peoples R China
3.Changshu Inst Technol, Coll Biol & Food Engn, Changshu, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
5.Jiangsu Univ, Sch Life Sci, Zhenjiang, Peoples R China
关键词： Lysobacter enzymogenes; photodegradation; macroporous resin adsorption; NKA resin; heat-stable antifungal factor
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Heat-stable antifungal factor (HSAF) is produced by the fermentation of Lysobacter enzymogenes, which is known for its broad-spectrum antifungal activity and novel mode of action. However, studies on the separation of HSAF have rarely been reported. Herein, alteramide B (the main byproduct) was removed firstly from the fermentation broth by photodegradation to improve the purity of HSAF. Then, the separation of HSAF via adsorption by macroporous adsorption resins (MARs) was evaluated and NKA resin showed highest static adsorption and desorption performances. After optimizing the static and dynamic adsorption characteristics, the content of HSAF in the purified product increased from 8.67 +/- 0.32% (ethyl acetate extraction) to 31.07 +/- 1.12% by 3.58-fold. These results suggest that the developed strategy via photodegradation and macroporous resin adsorption is an effective process for the separation of HSAF, and it is also a promising method for the large-scale preparation of HSAF for agricultural applications.
- 相关文献
作者其他论文 更多>>
-
First Report of Bacterial Fruit Blotch Caused by Kosakonia cowanii on Trichosanthis fructus in Jiangsu Province, China
作者：Chen, Xian;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Chen, Xian;Tang, Bao;Laborda, Pedro
关键词：bacterial fruit blotch; Kosakonia cowanii; Trichosanthis fructus
-
Unveiling the Role of Diffusible Signal Factor-Family Quorum Sensing Signals in Regulating Behavior of Xanthomonas and Lysobacter
作者：Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Mao, Yahui;Liu, Fengquan
关键词：diffusible signal factor; Lysobacter enzymogenes; quorum sensing; Xanthomonas campestris
-
S-nitrosylation of a receptor-like cytoplasmic kinase regulates plant immunity
作者：Cui, Beimi;Liu, Fengquan;Cui, Beimi;Liu, Fengquan;Cui, Beimi;Pan, Qiaona;Loake, Gary J.;Cui, Wenqiang;Wang, Yiqin;Cui, Wenqiang;Yuan, Shuguang;Wang, Yiqin;Loake, Verity I. P.;Loake, Gary J.
关键词：
-
Oomycete Nudix effectors display WY-Nudix conformation and mRNA decapping activity
作者：Guo, Baodian;Wang, Bangwei;Wang, Haonan;Kong, Guanghui;Dong, Suomeng;Wang, Yuanchao;Guo, Baodian;Wang, Bangwei;Liu, Fengquan;Hu, Qinli;Yao, Deqiang;Han, Chenyang;Liu, Fengquan;Xing, Weiman
关键词：
-
Evaluating the Efficacy of Hymexazol in Controlling Globisporangium spinosum, the Newly Identified Pathogen Causing Root Rot in Houttuynia cordata
作者：Zhou, Xue;Xiong, Chunlan;Ma, Chaoyun;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Zhou, Xue;Xiong, Chunlan;Ma, Chaoyun;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Tu, Yupei;Wang, Yong;Li, Kaihuai;Liu, Fengquan
关键词：Houttuynia cordata; hymexazol; oomycete; root rot
-
Regulatory Effects of Diverse DSF Family Quorum-Sensing Signals in Plant-Associated Bacteria
作者：Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Wang, Bo;Liu, Fengquan
关键词：bacterial intraspecies regulation; diffusible signal factor (DSF); plant innate immunity; quorum sensing (QS)
-
Uncovering the mechanisms underlying pear leaf apoplast protein-mediated resistance against Colletotrichum fructicola through transcriptome and proteome profiling
作者：Han, Chenyang;Su, Zhiyuan;Wang, Qi;Zhang, Shaoling;Zhao, Yancun;Li, Chaohui;Guo, Baodian;Liu, Fengquan
关键词：Colletotrichum fructicola; Pear; Apoplast; Glycoside hydrolases; Plant immunity
