文献类型： 外文期刊

作者： Li, Xiao ¹ ; Wang, Fen ¹ ; Liu, Mengqian ¹ ; Dong, Caihong ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Key Lab Edible Fungi Breeding, Guiyang 550000, Peoples R China

关键词： Cordyceps militaris; Cmhyd1; conidiation; hydrophobicity; infection; primordium differentiation; GATA transcription factor AreA; positive feedback

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.816； 五年影响因子：6.499 ）

ISSN：

年卷期： 2021 年 7 卷 8 期

页码：

收录情况： SCI

摘要： Hydrophobins are a family of small proteins exclusively secreted by fungi, and play a variety of roles in the life cycle. Cmhyd1, one of the hydrophobin class II members in Cordyceps militaris, has been shown to have a high transcript level during fruiting body development. Here, deletion of Cmhyd1 results in reduction in aerial mycelia, conidiation, hydrophobicity and infection ability, and complete inhibition of pigmentation and primordium differentiation. Cmhyd1 plays roles in conidiation and cuticle-bypassing infection by regulating the transcripts of frequency clock protein, Cmfrq, and velvet protein, Cmvosa, as well as primordium formation via the mitogen-activated protein kinase signaling pathway. Cmhyd1 also participates in stress response, including tolerance of mycelia to osmotic and oxidative stresses, and conidia to high or low temperatures. CmAreA, a transcription factor of nitrogen regulatory, is recruited to the promoter of Cmhyd1 and activates the transcription of Cmhyd1 with coactivator CmOTam using electrophoretic mobility shift assays and transient luciferase expression in tobacco. Furthermore, CmHYD1 is proved to regulate the transcription of Cmarea at different developmental stages via a positive feedback loop. These results reveal the diverse roles and regulation of Cmhyd1 in C. militaris, and provide insights into the developmental regulatory mechanism of mushrooms.