Development of a highly sensitive and selective electrochemical immunosensor for controlling of rhodamine B abuse in food samples
文献类型： 外文期刊
作者： Zhu, Lin 1 ; Dong, Xiu-Xiu 2 ; Gao, Chun-Bin 1 ; Gai, Zuoqi 3 ; He, Yong-Xin 4 ; Qian, Zhen-Jie 4 ; Liu, Yingju 1 ; Lei, Hong-Tao 1 ; Sun, Yuan-Ming 1 ; Xu, Zhen-Lin 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
2.Jiangsu Univ, Sch Agr Engn, Key Lab Modern Agr Equipment & Technol, Minist Educ, Zhenjiang 212013, Jiangsu, Peoples R China
3.Foshan Univ, Sch Life Sci & Engn, Foshan 528231, Peoples R China
4.Guangzhou Inst Food Control, Guangzhou 510410, Peoples R China
5.Chinese Acad Trop Agr Sci, Guangzhou Expt Stn, Guangzhou 510897, Peoples R China
关键词： Rhodamine B; Monoclonal antibody; Immunosensor; Cobalt hydroxide and gold nanoparticles; Cerium oxide
期刊名称：FOOD CONTROL （ 影响因子：6.652； 五年影响因子：6.498 ）
ISSN： 0956-7135
年卷期： 2022 年 133 卷
页码：
收录情况： SCI
摘要： Here, a highly sensitive and selective electrochemical immunosensor was established for the rapid screening of Rhodamine B (Rh B) in paprika and capsicol. Rh B complete antigen was prepared and used as immunogen to obtain anti-Rh B monoclonal antibody (anti-Rh B-mAb) through cell fusion technology. A layer of cobalt hydroxide and gold nanoparticles composite material (Co(OH)(2)-Au NPs) was modified on the surface of screenprinted electrode (SPE) by one-step electrodeposition. Subsequently, Rh B antigen was coated on the modified SPE as coating antigen, then Rh B and anti-Rh B-mAb labeled with cerium oxide (anti-Rh B-mAb@CeO2) were overlay simultaneously. Rh B can compete with the Rh B coating antigen to combine the anti-Rh B-mAb which causing electrical signal changes. After optimization of the assembled immunosensor, the assay showed a linear range when the concentration of Rh B ranged from 0.01 ng/mL to 10000 ng/mL. The average recovery rate of spiked samples was 85.7%similar to 106.8%, which was in good accordance with the standard HPLC determination. The immunosensor behaves high sensitivity and selectivity, and can be used in the rapid screening of Rh B in paprika and capsicol.
- 相关文献
作者其他论文 更多>>
-
Separation and Identification of Terpenoids in Three Pineapple Fibers Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
作者：Liu, Yijun;Chen, Gang;Liu, Yijun;Chen, Yuliang;Liu, Jiameng;Zhu, Lin;Lin, Lijing;Zhuang, Zhikai;He, Jiangxiu;Li, Tao;Yao, Siru;Lin, Lijing;Li, Tao;Chen, Gang
关键词：Terpenoids; pineapple fiber; separation; identification; ultra-performance liquid chromatography-tandem mass spectrometry
-
Preparation and characterization of tea tree essential oil microcapsule-coated packaging paper with bacteriostatic effect
作者：Zhu, Lin;Liu, Yijun;Liu, Jiameng;Qiu, Xunxia;Lin, Lijing;Zhu, Lin;Liu, Yijun;Liu, Jiameng;Qiu, Xunxia;Lin, Lijing
关键词：Antibacterial; Coated Kraft paper; Pullulan; Structural characterization; Tea tree essential oil microcapsule
-
Dynamic changes of microbiota and metabolite of traditional Hainan dregs vinegar during fermentation based on metagenomics and metabolomics
作者：Yu, Xiaohan;Guo, Xiaoxue;Guo, Ruijia;Zhu, Lin;Qiu, Xinrong;Chai, Jun;Liu, Fei;Gu, Chunhe;Feng, Zhen
关键词：Hainan dregs vinegar; Fermentation; Metagenomics; Volatile flavor compounds; Metabolomics
-
A simple and sensitive fluoroimmunoassay based on the nanobody-alkaline phosphatase fusion protein for the rapid detection of fenitrothion
作者：Chen, Zi-Jian;Huang, Ai-Jun;Wang, Hongwu;Chen, Zi-Jian;Huang, Ai-Jun;Wang, Hongwu;Chen, Zi-Jian;Huang, Ai-Jun;Wang, Hongwu;Dong, Xiu-Xiu;Zhang, Yi-Feng;Luo, Lin;Xu, Zhen-Lin;Zhu, Lin
关键词：fluoroimmunoassay; fenitrothion; carbon dots; nanobody-alkaline phosphatase fusion protein; rapid detection
-
A novel strategy for improving the antioxidant, iridoid, and flavor properties of Noni (Morinda citrifolia L.) fruit juice by lactic acid bacteria fermentation
作者：Zhang, Chao;Guo, Xiaoxue;Guo, Ruijia;Zhu, Lin;Qiu, Xinrong;Yu, Xiaohan;Chai, Jun;Feng, Zhen;Chen, Xiaoai;Gu, Chunhe;Feng, Zhen;Chen, Xiaoai;Gu, Chunhe;Feng, Zhen
关键词：Noni fruit juice; Lactic acid bacteria fermentation; Antioxidant; Iridoid; Flavor
-
Insights into the effects of extractable phenolic compounds and Maillard reaction products on the antioxidant activity of roasted wheat flours with different maturities
作者：Zhang, Chao;Guo, Xiaoxue;Guo, Ruijia;Zhu, Lin;Qiu, Xinrong;Yu, Xiaohan;Chai, Jun;Feng, Zhen;Gu, Chunhe;Feng, Zhen;Gu, Chunhe;Feng, Zhen
关键词：Immature wheat flours; Roasting; Phenolic compounds; Maillard reaction products; Antioxidant activity
-
Characterization of polyvinyl alcohol-nanocellulose composite film and its release effect on tetracycline hydrochloride
作者：Zhu, Lin;Feng, Lu;Wang, Ming-yan;Yao, Guanglong;Chen, Jian;Luo, Haixi;Dong, Rong-shu
关键词：Desmodium intortum(Mill.) Urb.; Nanocellulose; Polyvinyl alcohol composite film; Cytotoxicity
