Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application
文献类型： 外文期刊
作者： Wang, Zui 1 ; Liu, Lina 2 ; Dou, Junfeng 2 ; Li, Li 1 ; Lu, Qin 1 ; Jin, Xinxin 1 ; Shao, Huabin 2 ; Cheng, Zhengyu 2 ; Zhang, Tengfei 2 ; Luo, Qingping 2 ; Bei, Weicheng 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
2.Hubei Acad Agr Sci, Minist Agr & Rural Affairs, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Peoples R China
3.Hubei Hongshan Lab, Wuhan 430064, Peoples R China
4.Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS B3H 4R2, Canada
关键词： monoclonal antibody; avian leukosis virus; B-cell epitope
期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （ 2023影响因子：2.8； 五年影响因子：2.9 ）
ISSN： 1467-3037
年卷期： 2024 年 46 卷 6 期
收录情况： SCI
摘要： Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can impair immunological function, stunt growth and decrease egg production in avian flocks. The capsid protein (P27) is an attractive candidate for ALV diagnostics. In the present study, a new hybridoma cell (1F8) stably secreting an anti-P27 monoclonal antibody (mAb) was developed. The mAb exhibited a high affinity constant (Ka) of 8.65 x 106.0 L/mol, and it could be used for the detection of ALV-A/B/J/K strains. Moreover, a total of eight truncated recombinant proteins and five synthetic polypeptides were utilized for the identification of the B-cell epitopes present on P27. The results revealed that 218IIKYVLDRQK227 was the minimal epitope recognized by 1F8, which had never been reported before. Additionally, the epitopes could strongly react with different ALV subgroup's specific positive serum and had a complete homology among all the ALV subgroups strains. Finally, a new sandwich ELISA method was created for the detection of ALV antigens, demonstrating increased sensitivity compared to a commercially available ELISA kit. These results offer essential knowledge for further characterizing the antigenic composition of ALV P27 and will facilitate the development of diagnostic reagents for ALV.
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