文献类型： 外文期刊

作者： Sun, Yunhao ¹ ; Zheng, Chuanyuan ¹ ; Zhou, Jianuan ⁶ ; Zhen, Meng ¹ ; Wei, Xingying ¹ ; Yan, Xun ¹ ; Guo, Xiaojian ¹ ; Zheng, Li ¹ ; Shao, Mingwei ¹ ; Li, Chunji ¹ ; Qin, Di ¹ ; Zhang, Jie ¹ ; Xiong, Lina ⁷ ; Xing, Juejun ⁸ ; Huang, Bingzhi ⁹ ; Dong, Zhangyong ¹ ; Cheng, Ping ¹ ; Yu, Guohui ¹ ;

作者机构： 1.Zhongkai Univ Agr & Engn, Innovat Inst Plant Hlth, Guangzhou 510225, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Green Prevent & Control Fruits & Vegetable, Beijing, Peoples R China

3.Guangdong Univ Key Lab Sustainable Control Fruit &, Guangzhou 510225, Peoples R China

4.Zhongkai Univ Agr & Engn, Coll Agr & Biol, Guangzhou 510225, Peoples R China

5.Zhongkai Univ Agr & Engn, Coll Resources & Environm, Guangzhou 510225, Peoples R China

6.South China Agr Univ, Integrat Microbiol Res Ctr, Guangdong Prov Key Lab Microbial Signals & Dis Con, Guangzhou 510642, Peoples R China

7.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China

8.Zhongkai Univ Agr & Engn, Lab & Equipment Management Dept, Guangzhou 510225, Peoples R China

9.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510000, Peoples R China

关键词： banana disease; banana sheath rot; Fenza No.1; Klebsiella variicola; opportunistic pathogen

期刊名称：PLANT DISEASE （ 影响因子：4.5； 五年影响因子：5.0 ）

ISSN： 0191-2917

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Banana (Musa spp.) is an important fruit and food crop worldwide. In recent years, banana sheath rot has become a major problem in banana cultivation, causing plant death and substantial economic losses. Nevertheless, the pathogen profile of this disease has not been fully characterized. Klebsiella variicola is a versatile bacterium capable of colonizing different hosts, such as plants, humans, insects, and animals, and is recognized as an emerging pathogen in various hosts. In this study, we obtained 12 bacterial isolates from 12 different banana samples showing banana sheath rot in Guangdong and Guangxi Provinces, China. Phylogenetic analysis based on 16S rRNA sequences confirmed that all 12 isolates were K. variicola strains. We sequenced the genomes of these strains, performed comparative genomic analysis with other sequenced K. variicola strains, and found a lack of consistency in accessory gene content among these K. variicola strains. However, prediction based on the pan-genome of K. variicola revealed 22 unique virulence factors carried by the 12 pathogenic K. variicola isolates. Microbiome and microbial interaction network analysis of endophytes between the healthy tissues of diseased plants and healthy plants of two cultivars showed that Methanobacterium negatively interacts with Klebsiella in banana plants and that Herbaspirillum might indirectly inhibit Methanobacterium to promote Klebsiella growth. These results suggest that banana sheath rot is caused by the imbalance of plant endophytes and opportunistic pathogenic bacteria, providing an important basis for research and control of this disease.