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High ammonium inhibits root growth in Arabidopsis thaliana by promoting auxin conjugation rather than inhibiting auxin biosynthesis

文献类型： 外文期刊

作者： Di, Dong-Wei 1 ; Li, Guangjie 1 ; Sun, Li 1 ; Wu, Jingjing 1 ; Wang, Meng 1 ; Kronzucker, Herbert J. 4 ; Fang, Shuang; 1 ;

作者机构： 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China

2.Nanjing Agr Univ, Cytogenet Inst, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China

4.Univ British Columbia, Fac Land & Food Syst, Vancouver, BC V6T 1Z4, Canada

5.Univ Melbourne, Sch BioSci, Parkville, Vic 3010, Australia

6.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing 100049, Peoples R China

关键词： High-NH4+stress; IAA biosynthesis; IAA conjugation; Primary root growth inhibition; Elongation zone

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2021 年 261 卷

页码：

收录情况： SCI

摘要： Ammonium (NH4+) inhibits primary root (PR) growth in most plant species when present even at moderate concentrations. Previous studies have shown that transport of indole-3-acetic acid (IAA) is critical to maintaining root elongation under high-NH4+ stress. However, the precise regulation of IAA homeostasis under high-NH4+ stress (HAS) remains unclear. In this study, qRT-PCR, RNA-seq, free IAA and IAA conjugate and PR elongation measurements were conducted in genetic mutants to investigate the role of IAA biosynthesis and conjugation under HAS. Our data clearly show that HAS decreases free IAA in roots by increasing IAA inactivation but does not decrease IAA biosynthesis, and that the IAA-conjugating genes GH3.1, GH3.2, GH3.3, GH3.4, and GH3.6 function as the key genes in regulating high-NH4+ sensitivity in the roots. Furthermore, the analysis of promoter:: GUS staining in situ and genetic mutants reveals that HAS promotes IAA conjugation in the elongation zone (EZ), which may be responsible for the PR inhibition observed under HAS. This study provides potential new insight into the role of auxin in the improvement of tolerance to NH4+.

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