AMP-activated kinase regulates porcine reproductive and respiratory syndrome virus infection in vitro
文献类型： 外文期刊
作者： Fang, Jianyu 1 ; Wang, Hongkui 2 ; Lang, Limin 1 ; Li, Haili 1 ; Li, Shaoyu 1 ; Wang, Keling 1 ;
作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450002, Peoples R China
2.Henan Univ Anim Husb & Econ, Livestock Prod Qual Inspect Inst, Zhengzhou 450046, Peoples R China
关键词： AMP-activated protein kinase; Phosphorylation; Porcine reproductive and respiratory syndrome; Virus replication and infection
期刊名称：VIRUS GENES （ 影响因子：2.198； 五年影响因子：1.938 ）
ISSN： 0920-8569
年卷期： 2022 年 58 卷 2 期
页码：
收录情况： SCI
摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen in the pig industry worldwide. Many viruses manipulate their cellular metabolism to replicate themselves and cause infection. A conserved cellular energy sensor, 5'-AMP-activated protein kinase (AMPK), maintains cellular energy homeostasis. We found that PRRSV infection caused significant AMPK activation in a time-dependent manner via the ROS-calcium/calmodulin-dependent protein kinase-2 pathway. RNA interference-mediated AMPK knockdown could increase PRRSV replication in MARC-145 cells, suggesting that AMPK contributed to PRRSV infection regulation. Moreover, investigation of the effect of AMPK activity on PRRSV replication showed that PRRSV replication could be suppressed by the pharmacological agonists 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside and A769662. Conversely, an AMPK inhibitor, compound C, markedly enhanced PRRSV infection. Furthermore, the AMPK agonist A769662 was found to exert no effect on PRRSV entry, assembly, and release, suggesting that A769662 may hinder the PRRSV genome replication in MARC-145 cells. In conclusion, AMPK may be a promising antiviral drug target against PRRSV infection.
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