您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular Mechanism by Which the BrFIT2-BrbHLH-BrIRT1 Module Synergistically Regulates Iron Absorption in Brassica rapa ssp. Pekinensis

文献类型： 外文期刊

作者： Shen, Changwei 1 ; Qin, Yunduan 2 ; Li, Shuai 1 ; Chen, Ruixiang 2 ; Zhang, Xiaoli 4 ; Zhang, Yuanyuan 2 ; Feng, Chunyang 2 ; Xu, Yu 2 ; Yuan, Ranghua 5 ; Guo, Xinlei 2 ; Yuan, Jingping 2 ;

作者机构： 1.Henan Inst Sci & Technol, Sch Plant Protect & Environm, Sch Bee Sci, Xinxiang, Peoples R China

2.Henan Inst Sci & Technol, Sch Hort & Landscape Architecture, Xinxiang, Peoples R China

3.Henan Engn Res Ctr Dev & Utilizat Characterist Hor, Xinxiang, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Zhejiang Key Lab Digital Dry Land Crops, Hangzhou, Peoples R China

5.Xinxiang Acad Agr Sci, Vegetable Res Inst, Xinxiang, Peoples R China

关键词： Chinese cabbage; iron; molecular mechanism; overexpression

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）

ISSN： 1467-7644

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Iron is essential for plant photosynthesis and human health, yet Brassica rapa ssp. pekinensis exhibits significantly lower iron content compared to other leafy vegetables, and its iron uptake mechanisms are not well understood. This study elucidates the molecular mechanisms governing iron uptake by investigating the roles of the iron-regulated gene BrFIT2 (Bra011972) and the iron transport protein gene BrIRT1 (Bra013419) through yeast two-hybrid, bimolecular fluorescence complementation, genetic transformation and physiological-biochemical analyses, etc. Key findings include: BrFIT2 encodes a nuclear transcription factor containing an HLH domain, with its expression in roots upregulated 6.9-fold under iron-deficient conditions. Overexpression of BrFIT2 increased iron content in leaves and roots by 1.42- and 6.17-fold, respectively, whereas silencing BrFIT2 reduced leaf iron uptake by 60%-80%. Furthermore, BrFIT2 interacts with BrbHLH38/100 to form nuclear complexes that activate the expression of the iron transporter gene BrIRT1. Overexpression of BrIRT1 resulted in root iron levels 16.29-fold higher than those in wild-type plants. This study is the first to delineate the BrFIT2-BrbHLH-BrIRT1 regulatory cascade in Brassica rapa, challenging the conventional model of direct FIT-target gene interactions.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台