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Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease

文献类型： 外文期刊

作者： Zhao, Yun-Tao 1 ; Zhang, Kun 1 ; Zeng, Jian 1 ; Yin, Haowen 1 ; Zheng, Wenjing 1 ; Li, Rui 1 ; Ding, Anzi 2 ; Chen, Shaohong 1 ; Liu, You 1 ; Wu, Wenjin 2 ; Jing, Zhanxin 3 ;

作者机构： 1.Guangdong Ocean Univ, Coll Food Sci & Technol, Modern Biochem Expt Ctr, Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China

2.Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

3.Guangdong Ocean Univ, Coll Chem & Environm, 1 Hai-Da Rd, Zhanjiang 524088, Guangdong, Peoples R China

4.Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol, 5 Nanhu Ave,Hongshan Dist, Wuhan 430064, Hubei, Peoples R China

关键词： Magnetic; Hydrogel beads; Neutral protease; Immobilization

期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.705； 五年影响因子：3.989 ）

ISSN： 0141-0229

年卷期： 2022 年 157 卷

页码：

收录情况： SCI

摘要： Enzyme immobilization technology has a key role in improving the stability of enzyme reaction systems and biocatalyst utilization rates. In this study, polyvinyl alcohol/sodium alginate@Fe3O4 (PVA/SA@Fe3O4) magnetic immobilized-enzyme hydrogel beads were prepared. Their structure and morphology were characterized by scanning electron microscopy, surface area and porosity analyses, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and a vibrating sample magnetometer. The capability of PVA/SA@Fe3O4 to adsorb neutral protease was investigated with variations in composition, temperature, pH, stirring speed, enzyme concentration, and crosslinking concentration. The optimal parameters of the immobilization process were determined by response surface methodology (3% neutral protease, 4% crosslinking at 200 rpm, 45 degrees C and pH 7.2), under which an immobilization rate of 41.98 mg/g was obtained. The thermal stability, acid-base stability, and reusability of the immobilized enzyme were improved significantly. After seven cycles, the immobilized enzyme activity remained at 30.8% that of the initial enzyme activity. The results indicate that the immobilization of NP onto magnetic PVA/SA@Fe3O4 hydrogel beads improves enzyme efficiency, giving this process potential industrial applications.

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