An electrochemical platform based on a hemin-rGO-cMWCNTs modified aptasensor for sensitive detection of kanamycin
文献类型： 外文期刊
作者： Gao, Tianyi 1 ; Sun, Chong 2 ; Zhang, Nana 2 ; Huang, Yang 2 ; Zhu, Hongxing 2 ; Wang, Chunmei 4 ; Cao, Jinxuan 1 ; Wang, 1 ;
作者机构： 1.Ningbo Univ, Key Lab Anim Prot Food Proc Technol Zhejiang Prov, Ningbo 315211, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China
3.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Jiangsu, Peoples R China
4.Jiangsu Acad Agr Sci, Cent Lab, Nanjing 210014, Peoples R China
期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）
ISSN：
年卷期： 2021 年 11 卷 26 期
页码：
收录情况： SCI
摘要： Kanamycin (KANA) residue in meat is particularly harmful to public health and there is an urgent need to establish a fast, accurate and low-cost method to determinate KANA in food quality control. In this paper, hemin-reduced graphene oxide-carboxylated multiwalled carbon nanotubes (hemin-rGO-cMWCNTs) were designed and prepared, and the characteristics of hemin-rGO-cMWCNTs are presented. After that, an aptamer/hemin-rGO-cMWCNTs sensor for determination of KANA was developed. The electrochemical characteristics were studied by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the sensitive response of the aptasensor towards KANA presented a wide concentration range of 10(-9) to 10(-6) M and a low detection limit of 0.36 nM (S/N = 3). Meanwhile, the aptasensor showed prominent selectivity, high stability and acceptable reproducibility in the application of KANA detection. In addition, the aptasensor detection in real samples correlated well with that obtained by liquid chromatograph mass spectrometer (LCMS).
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