文献类型： 外文期刊

作者： Hu, Jiao ¹ ; Zhang, Qi ¹ ; Peng, Peipei ¹ ; Li, Rumeng ¹ ; Li, Jun ¹ ; Wang, Xiaoquan ¹ ; Gu, Min ¹ ; Hu, Zenglei ¹ ; Hu, Shunlin ¹ ; Liu, Xiaowen ¹ ; Mei, Mei ⁵ ; Jiao, Xinan ⁶ ; Peng, Daxin ¹ ; Liu, Xiufan ¹ ;

作者机构： 1.Yangzhou Univ, Sch Vet Med, Anim Infect Dis Lab, 48 East Wenhui Rd, Yangzhou 225009, Jiangsu, Peoples R China

2.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Jiangsu, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

4.Yangzhou Univ, Minist Agr China 26116120, Key Lab Prevent & Control Biol Hazard Factors Ani, Yangzhou, Jiangsu, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing, Peoples R China

6.Yangzhou Univ, Jiangsu Key Lab Zoonosis, Yangzhou, Jiangsu, Peoples R China

关键词： Virus-like particle; Baculovirus expression system; Sf9 insect cells; Mice; Chickens; Subunit vaccine

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.246； 五年影响因子：3.565 ）

ISSN： 0378-1135

年卷期： 2022 年 264 卷

页码：

收录情况： SCI

摘要： Currently, highly pathogenic avian influenza (HPAI) H7N9 viruses still pose a potential pandemic threat. Influenza virus-like particle (VLP) is one of the most promising vaccine strategies to complement traditional egg dependent vaccines. Here, we generated a H7N9 VLP vaccine candidate by baculovirus expression system and evaluated its efficacy in chickens and mice. The H7N9 VLP was produced through co-infection of Sf9 insect cells with three recombinant baculoviruses expressing individual HA, NA and M1 gene of the HPAI H7N9 virus A/ chicken/Guangdong/GD15/2016. Intramuscular immunization of the H7N9 VLP elicited robust antibody immune responses and conferred complete clinical protection against lethal H7N9 virus challenge both in chickens and mice. Meanwhile, H7N9 VLP significantly restrained virus shedding and dramatically alleviated pulmonary lesions caused by H7N9 virus infection in birds and mice. Interestingly, chicken antibodies induced by the H7N9 VLP also had a good cross-reactivity with H7N9 field strains isolated in different years. In addition, vaccination with the H7N9 VLP elicited high T cell immunity in mouse lung, evidenced by significantly upregulated expression of IL-2, IL-4 and IFN-gamma. Furthermore, the H7N9 VLP significantly decreased the expression of some key inflammatory cytokines, such as IL6, RANTES and TNF-alpha in mouse lung, which may partially account for its contribution to alleviate lung pathology. Therefore, our study describes the good efficacy of the HA + NA + M1 containing H7N9 VLP both in chicken and mice models, highlighting the potential of VLP-based vaccine as a critical alternative of traditional egg-based vaccine for control of H7N9 influenza virus in both humans and poultry.