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Overexpression of StBBX14 Enhances Cold Tolerance in Potato

文献类型： 外文期刊

作者： Zhang, Heng 1 ; Chen, Mingjun 2 ; Luo, Xiaobo 2 ; Song, Li 1 ; Li, Fei 2 ;

作者机构： 1.Guizhou Univ, Inst Agrobioengineering, Coll Life Sci,Key Lab Plant Resource Conservat & G, Collaborat Innovat Ctr Mt Ecol & Agrobioengineerin, Guiyang 550025, Peoples R China

2.Guizhou Prov Acad Agr Sci, Guizhou Inst Biotechnol, Guiyang 550003, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Crop Genet Resources & Germplasm Innovat K, Guiyang 550003, Peoples R China

关键词： potato; cold resistance; BBX gene; overexpression; transcriptome; WGCNA

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Potato (Solanum tuberosum L.) is an important food crop, but low temperature affects the potato growth and yield. In this study, the expression level of StBBX14 was significantly increased over 1 h and then gradually decreased under cold stress. The subcellular localization of the StBBX14 protein took place in the nucleus. The OE-StBBX14 transgenic lines showed less leaf damage and significantly lower electrolyte leakage compared with the WT under cold stress, indicating that the overexpression of StBBX14 in the potato enhanced the cold resistance. A transcriptome analysis showed that a total of 2449 and 6274 differentially expressed genes were identified in WT-1 h and WT-12 h, respectively, when compared with WT-0h. A Gene Ontology enrichment analysis revealed that photosynthesis, cell wall, thylakoid, transcription regulator activity, oxidoreductase activity and glucosyltransferase activity were significantly enriched in OE-StBBX14 and WT. A total of 14 distinct modules were generated by a WGCNA analysis based on all differentially expressed genes (DEGs). Four major modules with cold-related genes were isolated. RT-qPCR analysis showed that the expression patterns of eight DEGs were consistent between the qPCR and RNA-seq. These findings illustrate that the StBBX14 played an important role in cold stress in potato and provided a data basis for the genetic improvement of cold resistance traits of potato.

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