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The Antibacterial Activity and Mechanisms of a Mixed Bio-Preservative on the Bacillus Stains in Crab Roe Sauce

文献类型： 外文期刊

作者： Yu, Rongrong 1 ; Sun, Rongxue 2 ; Jiang, Ning 2 ; Zhang, Bin 1 ; Wang, Cheng 2 ; Liu, Qianyuan 2 ; Li, Zhiqiang 2 ; Wang, Xingna 2 ;

作者机构： 1.Zhejiang Ocean Univ, Coll Food & Pharm, Zhejiang Prov Key Lab Hlth Risk Factors Seafood, Zhoushan 316022, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China

3.Minist Agr & Rural Affairs, Integrated Sci Res Base Preservat Storage & Proc T, Nanjing 210014, Peoples R China

关键词： crab roe sauce; specific spoilage bacteria; epsilon-polylysine hydrochloride; nisin; tea polyphenols

期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）

ISSN：

年卷期： 2025 年 14 卷 3 期

页码：

收录情况： SCI

摘要： Crab roe sauce (CRS) is prone to spoilage due to microbial contamination. Therefore, this study aimed to investigate the inhibitory effects and mechanisms of a mixed bio-preservative (0.025% epsilon-polylysine hydrochloride (epsilon-PL) + 0.01% nisin (NS) + 0.01% tea polyphenols (TPs)) on the specific spoilage bacteria (SSB) in CRS. First, the SSB in CRS were isolated and identified by 16S rRNA sequencing. Two isolates were selected as representative strains based on their enzymatic spoilage potential and spoilage capability in CRS. By comparing the inhibition zones, epsilon-PL, NS, and TPs were selected from five conventional bio-preservatives (epsilon-PL, NS, TPs, grape seed extract (GSE), and rosemary extract (RE)) to prepare the mixed bio-preservative. The results showed that the minimum inhibitory concentration (MIC) of the mixed bio-preservative against Bacillus pumilus and Bacillus subtilis was 56.3 mu g/mL. The growth curves and cell viability tests revealed that the mixed bio-preservative reduced the viability of both strains. The conductivity, alkaline phosphatase activity, and nucleic acid and soluble protein leakage indicated that the mixed bio-preservative disrupted the integrity of the cell walls and membranes of the two isolates in a concentration-dependent manner. Scanning electron microscopy further confirmed the damage to the cell membranes of the two isolates by the mixed bio-preservative. Overall, the mixed bio-preservative exhibited excellently inhibitory effects on the SSB and could be a promising method for the preservation of CRS.

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