Effects of Different Cryoprotectants on Cryopreservation of Sperm from Noble Scallop Mimachlamys nobilis
文献类型： 外文期刊
作者： Li, Minghao 1 ; Fu, Zhengyi 1 ; Yu, Gang 1 ; Ma, Zhenhua 1 ;
作者机构： 1.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572018, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China
3.Sanya Trop Fisheries Res Inst, Hainan Engn Res Ctr Deep Sea Aquaculture & Proc, Sanya 572018, Peoples R China
4.Sanya Trop Fisheries Res Inst, Int Joint Res Ctr Conservat & Applicat Fishery Res, Sanya 572018, Peoples R China
5.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
6.Flinders Univ S Australia, Coll Sci & Engn, Adelaide 5001, Australia
关键词：
期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.8； 五年影响因子：2.8 ）
ISSN：
年卷期： 2025 年 13 卷 3 期
页码：
收录情况： SCI
摘要： In cryopreservation technology, the choice of cryoprotectant plays a crucial role in cell survival and function. Different types of cryoprotectants, each with unique protective mechanisms, mitigate cellular damage from ice crystal formation during freezing. This study investigated the effects of different types and concentrations of cryoprotectants on the cryopreservation efficacy of noble scallop Mimachlamys nobilis sperm. Six cryoprotectants were tested, including four permeable cryoprotectants (dimethyl sulfoxide (DMSO), ethylene glycerol (EG), propylene glycerol (PG), methanol (MET)) and two non-permeable cryoprotectants (trehalose (TRE), fetal bovine serum (FBS)). The results showed that permeable cryoprotectants, which penetrate the cell membrane, regulate the osmotic pressure inside and outside cells to reduce dehydration damage. Among them, 10% DMSO provided the best protection, significantly preserving sperm motility, velocity, and morphology. Non-permeable cryoprotectants, although unable to penetrate cells, stabilized the extracellular environment at higher concentrations (such as FBS). Additionally, MET and FBS exhibited enhanced protective effects with increasing concentration, indicating their potential in reducing sperm structural damage at higher concentrations. Morphological observations indicated that freezing caused varying degrees of structural damage to sperm, with flagellar integrity being crucial for motility. Overall, selecting an appropriate cryoprotectant and concentration is essential for the efficient cryopreservation of M. nobilis sperm, providing a valuable reference for conserving germplasm resources of marine species.
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