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Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping

文献类型： 外文期刊

作者： Yu, Ruisong 1 ; Dong, Shijuan 1 ; Chen, Bingqing 1 ; Liu, Yingjie 1 ; Li, Fengping 1 ; Si, Fusheng 1 ; Xie, Chunfang 1 ; Li, Zhen 1 ;

作者机构： 1.Shanghai Acad Agr Sci SAAS, Shanghai Engn Res Ctr Breeding Pig, Inst Anim Husb & Vet Sci, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

2.Shandong Freda Biotech Co Ltd, Jinan 276715, Peoples R China

3.Qingdao Haier Biotech Co Ltd, Qingdao 266000, Peoples R China

关键词： porcine epidemic diarrhea virus; spike protein; epitope mapping; antigenic alteration

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 7 期

页码：

收录情况： SCI

摘要： The spike protein (S) plays a crucial role in porcine epidemic diarrhea virus (PEDV) infection and induces neutralizing antibodies. Mutations of the S protein are supposed to provide the main antigenic shift leading to the antigenic escape of PEDVs. It is therefore a significant question how much accumulation of antigenic shift could lead to the antigenic escape of the variant PEDV. To provide an answer in the study, B cell epitopes (BCEs) on the S protein of the PEDV vaccine strain CV777 (S-CV777) and variant strain SD2014 (S-SD2014) were mapped using biosynthetic peptides and rabbit anti-PEDV S serum. Seventy-nine and 68 linear BCEs were identified from S-CV777 and S-SD2014, respectively. While 66.2% of the BCEs of S-SD2014 could be recognized by anti-S-CV777 serum and 67.1% of S-CV777 BCEs could be recognized by anti-S-SD2014 serum, more than 40% of the BCEs identified using anti-S-CV777 serum on S-CV777 could not be recognized by anti-S-SD2014 serum and vice versa. The completely shared BCEs took low percentages of 29.4% and 25.3% for S-SD2014 and S-CV777, respectively. These results indicate a low conservation of antigenicity of the S protein compared to a relatively high amino acid sequence similarity of 92.2% between the two strains. The study provided a BCE shift reference of PEDV antigenic escape and surveillance control.

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