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Introgression of clubroot resistant gene into Brassica oleracea L. from Brassica rapa based on homoeologous exchange

文献类型： 外文期刊

作者： Zhu, Mingzhao 1 ; Yang, Limei 1 ; Zhang, Yangyong 1 ; Zhuang, Mu 1 ; Ji, Jialei 1 ; Hou, Xilin 3 ; Li, Zhansheng 1 ; Han, Fengqing 1 ; Fang, Zhiyuan 1 ; Lv, Honghao 1 ; Wang, Yong 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Minist Agr, Key Lab Biol & Genet Improvement Hort Crops, Beijing 100081, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Vegetable Sci, Beijing 100097, Peoples R China

3.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Clubroot is a soil-borne disease in cabbage (Brassica oleracea L. var. capitata L.) caused by Plasmodiophora brassicae, which poses a great threat to cabbage production. However, clubroot resistance (CR) genes in Brassica rapa could be introduced into the cabbage via breeding to make it clubroot resistant. In this study, CR genes from B. rapa were introduced into the cabbage genome and the mechanism of gene introgression was explored. Two methods were used to create CR materials: (i) The fertility of CR Ogura CMS cabbage germplasms containing CRa was restored by using an Ogura CMS restorer. After cytoplasmic replacement and microspore culture, CRa-positive microspore individuals were obtained. (ii) Distant hybridization was performed between cabbage and B. rapa, which contained three CR genes (CRa, CRb, and Pb8.1). Finally, BC2 individuals containing all three CR genes were obtained. Inoculation results showed that both CRa-positive microspore individuals and BC2 individuals containing three CR genes were resistant to race 4 of P. brassicae. Sequencing results from CRa-positive microspore individuals with specific molecular markers and genome-wide association study (GWAS) showed penetration at the homologous position of the cabbage genome by a 3.42 Mb CRa containing a fragment from B. rapa; indicating homoeologous exchange (HE) as the theoretical basis for the introgression of CR resistance. The successful introduction of CR into the cabbage genome in the present study can provide useful clues for creating introgression lines within other species of interest.

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