Development of PAM-flexible multiplex genome editors for enhancing cold-tolerance in indica rice
文献类型： 外文期刊
作者： Tan, Jiantao 1 ; Sun, Kangli 1 ; Qian, Qian 1 ; Chai, Nan 2 ; Peng, Xin 1 ; Shen, Mengyuan 1 ; Zhou, Degui 1 ; Liu, Yao-Guang 2 ; Zhu, Qinlong 2 ; Liu, Qi 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Guangdong Rice Engn Lab,Rice Res Inst, Key Lab Genet & Breeding High Qual Rice Southern C, Minist Agr & Rural Affairs,Guangdong Key Lab Rice, Guangzhou 510640, Guangdong, Peoples R China
2.South China Agr Univ, Coll Agr, Guangdong Basic Res Ctr Excellence Precise Breedin, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Guangdong, Peoples R China
关键词： Genetic engineering; Genetic transformation; Genome editing; WRKY transcription factors
期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）
ISSN： 2095-5421
年卷期： 2025 年 13 卷 3 期
页码：
收录情况： SCI
摘要： The canonical CRISPR/Cas editors are constrained by the requirement for specific PAMs, which substantially limits their editable target range. Rice (particularly indica rice) is sensitive to low temperature, which impacts the yield and restricts the geographic distribution of rice. In this study, we developed PAM-flexible multiplex genome editing tools based on SpG (recognising NGN-PAMs) and SpRY (recognising NNN-PAMs) variants. We then tested the feasibility of using a sweet potato leaf curl virus (SPLCV) replicon-based expression vector and single-stranded DNA-binding domain (DBD) to improve the editing efficiency of these PAM-flexible editors. Furthermore, we used SpG-mediated multiplex genome editing to achieve comprehensive improvement in cold tolerance in indica rice by editing WRKY transcription factors OsWRKY53 and OsWRKY63, to generate high cold-resistant indica rice lines. We concluded that these PAM-flexible multiplex genome editors are powerful tools for multi-gene editing for crop genetic improvement. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- 相关文献
作者其他论文 更多>>
-
Genomic prediction with NetGP based on gene network and multi-omics data in plants
作者：Zhao, Longyang;Tang, Ping;Liu, Jianxiang;Xu, Zhi;Luo, Jinjing;Peng, Xin;Shen, Mengyuan;Wang, Chengrui;Zhao, Junliang;Zhou, Degui;Chen, Yibo;Liu, Qi;Peng, Xin;Shen, Mengyuan;Zhao, Junliang;Zhou, Degui;Chen, Yibo;Liu, Qi;Peng, Xin;Shen, Mengyuan;Zhao, Junliang;Zhou, Degui;Chen, Yibo;Liu, Qi;Peng, Xin;Shen, Mengyuan;Zhao, Junliang;Zhou, Degui;Chen, Yibo;Liu, Qi;Wang, Chengrui;Wang, Runfeng;Fan, Zhilan;Tang, Xiaoyan
关键词：genomic selection; feature selection; deep learning; multi-omics predictions; gene network
-
Comparative genomics uncovers evolutionary drivers of locust migratory adaptation
作者：Dong, Sujuan;Li, Xinghua;Tian, Aiwei;Chen, Nuo;Ban, Liping;Liu, Qi;Zhu, Tao;Tu, Xiongbing
关键词：Locust migration; Evolution; Positive selection; Metabolism; Mitochondria-related pathways
-
Improving yield-related traits by editing the promoter and distal regulatory region of heading date genes Ghd7 and PRR37 in elite rice variety Mei Xiang Zhan 2
作者：Guo, Xiaotong;Sun, Kangli;Wu, Zeqiang;Xiao, Dongdong;Song, Yingang;Li, Shengting;Wei, Guangliang;Li, Weitao;Hao, Yu;Xu, Bingqun;Zhang, Kai;Liao, Nan;Hu, Dan;Liu, Yao-Guang;Zong, Wubei;Guo, Jingxin;Sun, Kangli;Zong, Wubei
关键词：
-
Engineering Adenine Deaminase TadA for Precise and PAM-Flexible Point Mutagenesis and Gradient-Tuning Endogenous Protein Design
作者：Sun, Kangli;Qian, Qian;Chen, Ke;Peng, Xin;Shen, Mengyuan;Zhou, Degui;Liu, Qi;Tan, Jiantao;Chai, Nan;Zhang, Ruixiang;Zheng, Zhiye;Zhu, Qinlong;Cheng, Si;Mi, Jianing;Zeng, Dongchang
关键词：Badh2; gradient-tuned protein design; point mutagenesis; precise base editing; TadA variants
-
CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants
作者：Cai, Rongxiao;Zhang, Jiekun;Zeng, Dongchang;Chai, Nan;Liu, Yao-Guang;Zhu, Qinlong;Tan, Jiantao
关键词：
-
A new strategy of molecular breeding for optimal heading date and grain yield in rice by modulating elite allelic combinations of Ghd7, Hd3a, RFT1 and Gn1a
作者：Su, Qingmei;Zhang, Yuhang;Zhu, Li;Qian, Qian;Su, Qingmei;Zhao, Mengyue;Zhu, Guanlin;Zhang, Fang;Chen, Fan;Wang, Rongsheng;Qian, Qian;Chen, Fan;Feng, Xiaomin;Wang, Qing;Qian, Qian;Chen, Fan;Wang, Rongsheng;Feng, Xiaomin;Lin, Shaoyang;Zhang, Yuhang;Zhu, Li;Qian, Qian
关键词：Adaptation; Elite gene combinations; Oryza sativa; Photoperiod response
-
Rhodopseudomonas palustris shapes bacterial community, reduces Cd bioavailability in Cd contaminated flooding paddy soil, and improves rice performance
作者：Su, Yanqiu;Su, Yanqiu;Li, Ziyuan;Deng, Hongmei;Zhou, Qian;Li, Lihuan;Zhao, Lanyin;Shi, Qiuyun;Chen, Yanger;Yuan, Shu;Liu, Qi
关键词：Photosynthetic bacteria; Rhodopseudomonas palustris; Cadmium; Oryza sativa L.; Anaerobic bacteria
